Plasmid-Based One-Pot Saturation Mutagenesis and Robot-Based Automated Screening for Protein Engineering
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DOI: 10.1021/acsomega.8b00663
Abstract: We evaluated a method for protein engineering using plasmid-based one-pot saturation mutagenesis and robot-based automated screening. When the biases in nucleotides and amino acids were assessed for a loss-of-function point mutation in green fluorescent protein,… read more here.
Keywords: protein; pot saturation; protein engineering; plasmid based ... See more keywords