Missing the Mark: PRDM9-Dependent Methylation Is Required for Meiotic DSB Targeting.
Sign Up to like & getrecommendations! Published in 2018 at "Molecular cell"
DOI: 10.1016/j.molcel.2018.02.021
Abstract: PRDM9 determines the localization of meiotic recombination hotspots, which are associated with histone H3 methylation. It is not known whether PRDM9's methyltransferase activity is required or how some PRDM9 alleles can dominate the distribution of hotspots… read more here.
Keywords: methylation; missing mark; mark prdm9; prdm9 ... See more keywords
PRDM9 losses in vertebrates are coupled to those of paralogs ZCWPW1 and ZCWPW2
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2114401119
Abstract: Significance We take a phylogenetic approach to search for molecular partners of PRDM9, a key meiotic recombination gene, by leveraging the fact that the complete PRDM9 gene has been lost at least 13 times independently… read more here.
Keywords: prdm9 losses; coupled paralogs; vertebrates coupled; paralogs zcwpw1 ... See more keywords
In vivo binding of PRDM9 reveals interactions with noncanonical genomic sites.
Sign Up to like & getrecommendations! Published in 2017 at "Genome research"
DOI: 10.1101/gr.217240.116
Abstract: In mouse and human meiosis, DNA double-strand breaks (DSBs) initiate homologous recombination and occur at specific sites called hotspots. The localization of these sites is determined by the sequence-specific DNA binding domain of the PRDM9… read more here.
Keywords: vivo binding; binding prdm9; prdm9; genomic sites ... See more keywords
Meiotic Epigenetic Factor PRDM9 Impacts Sperm Quality of Hybrid Mice.
Sign Up to like & getrecommendations! Published in 2020 at "Reproduction"
DOI: 10.1530/rep-19-0528
Abstract: Reduced fertility of male mouse hybrids relative to their parents, or hybrid sterility, is governed by the hybrid sterility 1 (Hst1) locus. Rescue experiments with transgenes carrying sequences within or near Hst1 manifested that Hst1… read more here.
Keywords: sperm quality; meiotic epigenetic; epigenetic factor; factor prdm9 ... See more keywords
The KRAB Domain of ZNF10 Guides the Identification of Specific Amino Acids That Transform the Ancestral KRAB-A-Related Domain Present in Human PRDM9 into a Canonical Modern KRAB-A Domain
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23031072
Abstract: Krüppel-associated box (KRAB) zinc finger proteins are a large class of tetrapod transcription factors that usually exert transcriptional repression through recruitment of TRIM28/KAP1. The evolutionary root of modern KRAB domains (mKRAB) can be traced back… read more here.
Keywords: krab domain; domain; modern krab; prdm9 ... See more keywords
A map of human PRDM9 binding provides evidence for novel behaviors of PRDM9 and other zinc-finger proteins in meiosis
Sign Up to like & getrecommendations! Published in 2017 at "eLife"
DOI: 10.7554/elife.28383
Abstract: PRDM9 binding localizes almost all meiotic recombination sites in humans and mice. However, most PRDM9-bound loci do not become recombination hotspots. To explore factors that affect binding and subsequent recombination outcomes, we mapped human PRDM9… read more here.
Keywords: prdm9 zinc; human prdm9; prdm9; recombination ... See more keywords
PRDM9 activity depends on HELLS and promotes local 5-hydroxymethylcytosine enrichment
Sign Up to like & getrecommendations! Published in 2020 at "eLife"
DOI: 10.7554/elife.57117
Abstract: Meiotic recombination starts with the formation of DNA double-strand breaks (DSBs) at specific genomic locations that correspond to PRDM9-binding sites. The molecular steps occurring from PRDM9 binding to DSB formation are unknown. Using proteomic approaches… read more here.
Keywords: hydroxymethylcytosine; enrichment; prdm9 binding; dsb activity ... See more keywords