Nuclear export of the pre-60S ribosomal subunit through single nuclear pores observed in real time
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DOI: 10.1038/s41467-021-26323-7
Abstract: Ribosomal biogenesis has been studied by biochemical, genetic and electron microscopic approaches, but live cell data on the in vivo kinetics are still missing. Here we analyse the export kinetics of the large ribosomal subunit… read more here.
Keywords: export; nuclear export; ribosomal subunit; pre 60s ... See more keywords
Rbp95 binds to 25S rRNA helix H95 and cooperates with the Npa1 complex during early pre-60S particle maturation
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac724
Abstract: Abstract Eukaryotic ribosome synthesis involves more than 200 assembly factors, which promote ribosomal RNA (rRNA) processing, modification and folding, and assembly of ribosomal proteins. The formation and maturation of the earliest pre-60S particles requires structural… read more here.
Keywords: maturation; pre 60s; early pre; pre ... See more keywords
Viewing pre-60S maturation at a minute’s timescale
Sign Up to like & getrecommendations! Published in 2018 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkx1293
Abstract: Abstract The formation of ribosomal subunits is a highly dynamic process that is initiated in the nucleus and involves more than 200 trans-acting factors, some of which accompany the pre-ribosomes into the cytoplasm and have… read more here.
Keywords: viewing pre; maturation minute; minute timescale; 60s maturation ... See more keywords
The L1 stalk is required for efficient export of nascent large ribosomal subunits in yeast.
Sign Up to like & getrecommendations! Published in 2019 at "RNA"
DOI: 10.1261/rna.071811.119
Abstract: The ribosomal protein Rpl1 (uL1 in universal nomenclature) is essential in yeast and constitutes part of the L1 stalk which interacts with E site ligands on the ribosome. Structural studies of nascent pre-60S complexes in… read more here.
Keywords: export; ribosomal subunits; required efficient; stalk required ... See more keywords
Mechanism of 5S RNP recruitment and helicase-surveilled rRNA maturation during pre-60S biogenesis.
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DOI: 10.15252/embr.202356910
Abstract: Ribosome biogenesis proceeds along a multifaceted pathway from the nucleolus to the cytoplasm that is extensively coupled to several quality control mechanisms. However, the mode by which 5S ribosomal RNA is incorporated into the developing… read more here.
Keywords: biogenesis; mechanism rnp; maturation; pre 60s ... See more keywords
Mutational Analyses of the Cysteine-Rich Domain of Yvh1, a Protein Required for Translational Competency in Yeast
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DOI: 10.3390/biology11081246
Abstract: Simple Summary Ribosome maturation in eukaryotes is a highly energy-consuming, evolutionarily conserved process, directed by a large number of auxiliary proteins (>200) called trans-acting factors (TAFs). TAFs direct the ordered and directional assembly of immature… read more here.
Keywords: yvh1; 60s complexes; pre 60s; domain ... See more keywords