Retroelement origins of pre‐mRNA splicing
Sign Up to like & getrecommendations! Published in 2020 at "Wiley Interdisciplinary Reviews: RNA"
DOI: 10.1002/wrna.1589
Abstract: Recent cryo‐EM structures of a group II intron caught in the process of invading DNA have given new insight into the mechanisms of both splicing and retrotransposition. Conformational dynamics involving the branch‐site helix domain VI… read more here.
Keywords: group; origins pre; mrna splicing; pre mrna ... See more keywords
Design and In Vitro Use of Antisense Oligonucleotides to Correct Pre-mRNA Splicing Defects in Inherited Retinal Dystrophies.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7522-8_5
Abstract: Antisense oligonucleotides (AONs) are small molecules able to bind to the pre-mRNA and modulate splicing. The increasing amount of intronic mutations leading to pseudoexon insertion in genes underlying inherited retinal dystrophies (IRDs) has highlighted the… read more here.
Keywords: correct pre; retinal dystrophies; mrna splicing; pre mrna ... See more keywords
Binding of SRSF4 to a novel enhancer modulates splicing of exon 6 of Fas pre-mRNA.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2018.10.123
Abstract: Alternative splicing of exon 6 in Fas pre-mRNA generates a membrane bound pro-apoptotic isoform or soluble anti-apoptotic isoform. SRSF4 is a member of Arginine-Serine rich (SR) protein family. Here we demonstrate that increased SRSF4 expression… read more here.
Keywords: pre mrna; srsf4; fas pre; splicing exon ... See more keywords
A synthetic small molecule stalls pre-mRNA splicing by promoting an early-stage U2AF2-RNA complex.
Sign Up to like & getrecommendations! Published in 2021 at "Cell chemical biology"
DOI: 10.1016/j.chembiol.2021.02.007
Abstract: Dysregulated pre-mRNA splicing is an emerging Achilles heel of cancers and myelodysplasias. To expand the currently limited portfolio of small-molecule drug leads, we screened for chemical modulators of the U2AF complex, which nucleates spliceosome assembly… read more here.
Keywords: small molecule; mrna splicing; pre mrna; u2af2 rna ... See more keywords
Specific intron-dependent loading of DAZAP1 onto the cox6c transcript suppresses pre-mRNA splicing efficacy and induces cell growth retardation.
Sign Up to like & getrecommendations! Published in 2018 at "Gene"
DOI: 10.1016/j.gene.2018.03.005
Abstract: DAZAP1 is an evolutionarily conserved RNA-binding protein expressed in many tissues in mice and humans. DAZAP1-knockout mice carrying a partial loss-of-function (hypomorphic) allele exhibited severe deficiencies in spermatogenesis and cell growth, indicating that DAZAP1 plays… read more here.
Keywords: cox6c; cell growth; mrna splicing; dazap1 ... See more keywords
The G-Quadruplex-Specific RNA Helicase DHX36 Regulates p53 Pre-mRNA 3'-End Processing Following UV-Induced DNA Damage.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of molecular biology"
DOI: 10.1016/j.jmb.2016.11.033
Abstract: Pre-mRNA 3'-end processing, the process through which almost all eukaryotic mRNAs acquire a poly(A) tail is generally inhibited during the cellular DNA damage response leading to a profound impact on the level of protein expression… read more here.
Keywords: mrna end; dna damage; end processing; pre mrna ... See more keywords
Box C/D snoRNP Autoregulation by a cis-Acting snoRNA in the NOP56 Pre-mRNA.
Sign Up to like & getrecommendations! Published in 2018 at "Molecular cell"
DOI: 10.1016/j.molcel.2018.08.017
Abstract: Box C/D snoRNAs constitute a class of abundant noncoding RNAs that associate with common core proteins to form catalytic snoRNPs. Most of these operate in trans to assist the maturation of rRNAs by guiding and… read more here.
Keywords: nop56 pre; snorna; box; box snornp ... See more keywords
Pseudouridine synthases modify human pre-mRNA co-transcriptionally and affect pre-mRNA processing.
Sign Up to like & getrecommendations! Published in 2022 at "Molecular cell"
DOI: 10.1016/j.molcel.2021.12.023
Abstract: Pseudouridine is a modified nucleotide that is prevalent in human mRNAs and is dynamically regulated. Here, we investigate when in their life cycle mRNAs become pseudouridylated to illuminate the potential regulatory functions of endogenous mRNA… read more here.
Keywords: pseudouridine synthases; pre; mrna processing; pre mrna ... See more keywords
Emerging Roles of Ubiquitin-like Proteins in Pre-mRNA Splicing.
Sign Up to like & getrecommendations! Published in 2018 at "Trends in biochemical sciences"
DOI: 10.1016/j.tibs.2018.09.001
Abstract: Ubiquitin-like proteins (UBLs) belong to the protein family whose members share a globular beta-grasp fold structure. The archetypal member, ubiquitin, is known for its function in proteasome-mediated protein degradation. UBLs have been shown to play… read more here.
Keywords: like proteins; ubiquitin like; emerging roles; mrna splicing ... See more keywords
Understanding pre-mRNA splicing through crystallography.
Sign Up to like & getrecommendations! Published in 2017 at "Methods"
DOI: 10.1016/j.ymeth.2017.04.023
Abstract: Crystallography is a powerful tool to determine the atomic structures of proteins and RNAs. X-ray crystallography has been used to determine the structure of many splicing related proteins and RNAs, making major contributions to our… read more here.
Keywords: mrna splicing; understanding pre; crystallography; pre mrna ... See more keywords
The effects of structure on pre-mRNA processing and stability.
Sign Up to like & getrecommendations! Published in 2017 at "Methods"
DOI: 10.1016/j.ymeth.2017.06.001
Abstract: Pre-mRNA molecules can form a variety of structures, and both secondary and tertiary structures have important effects on processing, function and stability of these molecules. The prediction of RNA secondary structure is a challenging problem… read more here.
Keywords: secondary structure; structure pre; structure; pre mrna ... See more keywords