Modulation of DNA double-strand break repair as a strategy to improve precise genome editing
Sign Up to like & getrecommendations! Published in 2020 at "Oncogene"
DOI: 10.1038/s41388-020-01445-2
Abstract: In the present day, it is possible to incorporate targeted mutations or replace a gene using genome editing techniques such as customisable CRISPR/Cas9 system. Although induction of DNA double-strand breaks (DSBs) by genome editing tools… read more here.
Keywords: genome editing; double strand; dna double; precise genome ... See more keywords
Precise genome-wide mapping of single nucleosomes and linkers in vivo
Sign Up to like & getrecommendations! Published in 2018 at "Genome Biology"
DOI: 10.1186/s13059-018-1398-0
Abstract: We developed a chemical cleavage method that releases single nucleosome dyad-containing fragments, allowing us to precisely map both single nucleosomes and linkers with high accuracy genome-wide in yeast. Our single nucleosome positioning data reveal that… read more here.
Keywords: single nucleosomes; genome wide; wide mapping; nucleosomes linkers ... See more keywords
Improving Precise Genome Editing Using Donor DNA/gRNA Hybrid Duplex Generated by Complementary Bases
Sign Up to like & getrecommendations! Published in 2022 at "Biomolecules"
DOI: 10.3390/biom12111621
Abstract: In precise genome editing, site-specific DNA double-strand breaks (DSBs) induced by the CRISPR/Cas9 system are repaired via homology-directed repair (HDR) using exogenous donor DNA templates. However, the low efficiency of HDR-mediated genome editing is a… read more here.
Keywords: genome editing; dna; donor dna; efficiency ... See more keywords
Precise Genome Editing in Poultry and Its Application to Industries
Sign Up to like & getrecommendations! Published in 2020 at "Genes"
DOI: 10.3390/genes11101182
Abstract: Poultry such as chickens are valuable model animals not only in the food industry, but also in developmental biology and biomedicine. Recently, precise genome-editing technologies mediated by the CRISPR/Cas9 system have developed rapidly, enabling the… read more here.
Keywords: genome editing; genome edited; poultry application; precise genome ... See more keywords
Rapid and precise genome engineering in a naturally short-lived vertebrate
Sign Up to like & getrecommendations! Published in 2022 at "eLife"
DOI: 10.7554/elife.80639
Abstract: The African turquoise killifish is a powerful vertebrate system to study complex phenotypes at scale, including aging and age-related disease. Here we develop a rapid and precise CRISPR/Cas9-mediated knock-in approach in the killifish. We show… read more here.
Keywords: rapid precise; genome engineering; short lived; engineering naturally ... See more keywords