Time-Resolved Quantification of Nanoparticle Uptake, Distribution, and Impact in Precision-Cut Liver Slices.
Sign Up to like & getrecommendations! Published in 2020 at "Small"
DOI: 10.1002/smll.201906523
Abstract: Much effort within the nanosafety field is currently focused on the use of advanced in vitro models to reduce the gap between in vitro and in vivo studies. Within this context, precision-cut tissue slices are… read more here.
Keywords: impact; precision cut; nanoparticle; tissue ... See more keywords
Imaging Precision-Cut Lung Slices to Visualize Leukocyte Localization and Trafficking.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7896-0_18
Abstract: Pulmonary dendritic cells (DCs) are potent antigen-presenting cells that can activate both naïve and memory/effector T cells. However, very little is known of how movements and localization of DCs contribute to these events. To study… read more here.
Keywords: cut lung; precision cut; lung slices; localization ... See more keywords
Transcriptomic characterization of culture-associated changes in murine and human precision-cut tissue slices
Sign Up to like & getrecommendations! Published in 2019 at "Archives of Toxicology"
DOI: 10.1007/s00204-019-02611-6
Abstract: Our knowledge of complex pathological mechanisms underlying organ fibrosis is predominantly derived from animal studies. However, relevance of animal models for human disease is limited; therefore, an ex vivo model of human precision-cut tissue slices… read more here.
Keywords: human precision; cut tissue; precision cut; pathology ... See more keywords
Precision cut lung slices: an ex vivo model for assessing the impact of immunomodulatory therapeutics on lung immune responses.
Sign Up to like & getrecommendations! Published in 2021 at "Archives of toxicology"
DOI: 10.1007/s00204-021-03096-y
Abstract: Chronic inflammatory diseases of the respiratory tract, such as chronic obstructive pulmonary disease (COPD) and asthma, are severe lung diseases that require effective treatments. In search for new medicines for these diseases, there is an… read more here.
Keywords: cut lung; precision cut; vivo model; lung slices ... See more keywords
siRNA‐mediated protein knockdown in precision‐cut lung slices
Sign Up to like & getrecommendations! Published in 2018 at "European Journal of Pharmaceutics and Biopharmaceutics"
DOI: 10.1016/j.ejpb.2018.11.005
Abstract: &NA; Small interfering RNA (siRNA) can induce RNA interference, which leads to the knockdown of messenger RNA (mRNA) and protein. As a result, siRNA is often used in vitro and in vivo to unravel the… read more here.
Keywords: protein knockdown; sirna; precision cut; lung slices ... See more keywords
Design of a versatile microfluidic device for imaging precision-cut-tissue slices
Sign Up to like & getrecommendations! Published in 2022 at "Biofabrication"
DOI: 10.1088/1758-5090/ac7eea
Abstract: Precision-cut-tissues (PCTs), which preserve many aspects of a tissue’s microenvironment, are typically imaged using conventional sample dishes and chambers. These can require large amounts of reagent and, when used for flow-through experiments, the shear forces… read more here.
Keywords: versatile microfluidic; microfluidic device; design versatile; design ... See more keywords
Cryopreserved human precision-cut lung slices provide an immune competent pulmonary test system for “on-demand” use and long-term cultures
Sign Up to like & getrecommendations! Published in 2023 at "Toxicological Sciences"
DOI: 10.1093/toxsci/kfac136
Abstract: Abstract Human precision-cut lung slices (hPCLS), considered a highly relevant ex vivo model of the lung, offer native architecture and cells of the lung tissue including respiratory parenchyma, small airways, and immune competent cells. However,… read more here.
Keywords: cut lung; lung slices; lung; human precision ... See more keywords
Precision-cut lung slices: a powerful ex vivo model to investigate respiratory infectious diseases.
Sign Up to like & getrecommendations! Published in 2021 at "Molecular microbiology"
DOI: 10.1111/mmi.14817
Abstract: Respiratory infections are leading causes of mortality worldwide. Most of the research on the underlying disease mechanisms is based on cell culture, organoid or surrogate animal models. Although these provide important insights, they have limitations.… read more here.
Keywords: cut lung; precision cut; lung slices; respiratory ... See more keywords
Multiscale stiffness of human emphysematous precision cut lung slices
Sign Up to like & getrecommendations! Published in 2023 at "Science Advances"
DOI: 10.1126/sciadv.adf2535
Abstract: Emphysema is a debilitating disease that remodels the lung leading to reduced tissue stiffness. Thus, understanding emphysema progression requires assessing lung stiffness at both the tissue and alveolar scales. Here, we introduce an approach to… read more here.
Keywords: precision cut; cut lung; stiffness; lung ... See more keywords
Precision cut lung slices as a preclinical model for non-small cell lung cancer chemoprevention.
Sign Up to like & getrecommendations! Published in 2023 at "Cancer prevention research"
DOI: 10.1158/1940-6207.capr-23-0004
Abstract: Lung cancer chemoprevention is critical to addressing cancer burden in high-risk populations. Chemoprevention clinical trials rely on data from pre-clinical models however, in vivo studies have high financial, technical, and staffing requirements. Precision cut lung… read more here.
Keywords: lung cancer; cancer chemoprevention; lung; chemoprevention ... See more keywords
Precision-cut bovine udder slices (PCBUS) as an in-vitro-model of an early phase of infection of bovine mastitis
Sign Up to like & getrecommendations! Published in 2021 at "BMC Veterinary Research"
DOI: 10.1186/s12917-021-02817-w
Abstract: Background The aim of this study was to establish precision-cut bovine udder slices (PCBUS) as an in-vitro-model to investigate pathophysiological processes in the early phase of mastitis in order to have the possibility to investigate… read more here.
Keywords: vitro model; bovine; precision cut; model ... See more keywords