Time-Resolved Quantification of Nanoparticle Uptake, Distribution, and Impact in Precision-Cut Liver Slices.
Sign Up to like & getrecommendations! Published in 2020 at "Small"
DOI: 10.1002/smll.201906523
Abstract: Much effort within the nanosafety field is currently focused on the use of advanced in vitro models to reduce the gap between in vitro and in vivo studies. Within this context, precision-cut tissue slices are… read more here.
Keywords: impact; precision cut; nanoparticle; tissue ... See more keywords
Imaging Precision-Cut Lung Slices to Visualize Leukocyte Localization and Trafficking.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7896-0_18
Abstract: Pulmonary dendritic cells (DCs) are potent antigen-presenting cells that can activate both naïve and memory/effector T cells. However, very little is known of how movements and localization of DCs contribute to these events. To study… read more here.
Keywords: cut lung; precision cut; lung slices; localization ... See more keywords
Transcriptomic characterization of culture-associated changes in murine and human precision-cut tissue slices
Sign Up to like & getrecommendations! Published in 2019 at "Archives of Toxicology"
DOI: 10.1007/s00204-019-02611-6
Abstract: Our knowledge of complex pathological mechanisms underlying organ fibrosis is predominantly derived from animal studies. However, relevance of animal models for human disease is limited; therefore, an ex vivo model of human precision-cut tissue slices… read more here.
Keywords: human precision; cut tissue; precision cut; pathology ... See more keywords
Precision cut lung slices: an ex vivo model for assessing the impact of immunomodulatory therapeutics on lung immune responses.
Sign Up to like & getrecommendations! Published in 2021 at "Archives of toxicology"
DOI: 10.1007/s00204-021-03096-y
Abstract: Chronic inflammatory diseases of the respiratory tract, such as chronic obstructive pulmonary disease (COPD) and asthma, are severe lung diseases that require effective treatments. In search for new medicines for these diseases, there is an… read more here.
Keywords: cut lung; precision cut; vivo model; lung slices ... See more keywords
siRNA‐mediated protein knockdown in precision‐cut lung slices
Sign Up to like & getrecommendations! Published in 2018 at "European Journal of Pharmaceutics and Biopharmaceutics"
DOI: 10.1016/j.ejpb.2018.11.005
Abstract: &NA; Small interfering RNA (siRNA) can induce RNA interference, which leads to the knockdown of messenger RNA (mRNA) and protein. As a result, siRNA is often used in vitro and in vivo to unravel the… read more here.
Keywords: protein knockdown; sirna; precision cut; lung slices ... See more keywords
Free radical detection in precision-cut mouse liver slices with diamond-based quantum sensing
Sign Up to like & getrecommendations! Published in 2024 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2317921121
Abstract: Significance Here, we show diamond-based quantum sensing in tissue slices. We demonstrated this in precision cut liver slices which can be maintained alive for several days. This method allows measurements of free radical generation at… read more here.
Keywords: based quantum; quantum sensing; liver slices; precision cut ... See more keywords
Exploring precision-cut liver slices for comparative xenobiotic metabolism profiling in swine and cattle
Sign Up to like & getrecommendations! Published in 2024 at "Xenobiotica"
DOI: 10.1080/00498254.2024.2343905
Abstract: Abstract In vitro systems are useful tools for unravelling species differences in xenobiotic metabolism. The current work aimed to validate the technique of precision-cut liver slices (PCLS) for comparative studies on xenobiotic metabolism in swine… read more here.
Keywords: cut liver; swine cattle; oxygenation; precision cut ... See more keywords
Small RNA-based formulations in Precision cut lung slices - is there an alternative for in vivo/in vitro studies?
Sign Up to like & getrecommendations! Published in 2025 at "Expert review of respiratory medicine"
DOI: 10.1080/17476348.2025.2600109
Abstract: INTRODUCTION Precision cut lung slices (PCLS) are ex vivo models to study lung diseases. They have numerous advantages related to the presence of different cell types and extracellular matrix. They are an excellent platform to… read more here.
Keywords: lung slices; based formulations; rna based; small rna ... See more keywords
Design of a versatile microfluidic device for imaging precision-cut-tissue slices
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DOI: 10.1088/1758-5090/ac7eea
Abstract: Precision-cut-tissues (PCTs), which preserve many aspects of a tissue’s microenvironment, are typically imaged using conventional sample dishes and chambers. These can require large amounts of reagent and, when used for flow-through experiments, the shear forces… read more here.
Keywords: versatile microfluidic; microfluidic device; design versatile; design ... See more keywords
Cryopreserved human precision-cut lung slices provide an immune competent pulmonary test system for “on-demand” use and long-term cultures
Sign Up to like & getrecommendations! Published in 2023 at "Toxicological Sciences"
DOI: 10.1093/toxsci/kfac136
Abstract: Abstract Human precision-cut lung slices (hPCLS), considered a highly relevant ex vivo model of the lung, offer native architecture and cells of the lung tissue including respiratory parenchyma, small airways, and immune competent cells. However,… read more here.
Keywords: cut lung; lung slices; lung; human precision ... See more keywords
Precision-cut lung slices: a powerful ex vivo model to investigate respiratory infectious diseases.
Sign Up to like & getrecommendations! Published in 2021 at "Molecular microbiology"
DOI: 10.1111/mmi.14817
Abstract: Respiratory infections are leading causes of mortality worldwide. Most of the research on the underlying disease mechanisms is based on cell culture, organoid or surrogate animal models. Although these provide important insights, they have limitations.… read more here.
Keywords: cut lung; precision cut; lung slices; respiratory ... See more keywords