Repurposing Macromolecule Delivery Tools for Plant Genetic Modification in the Era of Precision Genome Engineering.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8778-8_1
Abstract: Efficient delivery of macromolecules into plant cells and tissues is important for both basic research and biotechnology product applications. In transgenic research, the goal is to deliver DNA molecules into regenerable cells and stably integrate… read more here.
Keywords: plant; delivery; era precision; macromolecule delivery ... See more keywords
Precision genome engineering through adenine and cytosine base editing
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DOI: 10.1038/s41477-018-0115-z
Abstract: Adenine base editors (ABEs), composed of an engineered deaminase and a catalytically impaired CRISPR–Cas9 variant, are powerful new tools for targeted base editing in cells and organisms. Together with cytosine base editors (CBEs), ABEs enable… read more here.
Keywords: base editing; engineering adenine; base; cytosine base ... See more keywords
Precision genome editing in the eye
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2210104119
Abstract: CRISPR-Cas-based genome editing technologies could, in principle, be used to treat a wide variety of inherited diseases, including genetic disorders of vision. Programmable CRISPR-Cas nucleases are effective tools for gene disruption, but they are poorly… read more here.
Keywords: genome editing; precision genome; genetic disorders; editing eye ... See more keywords
CRISPR/Cas‐based precision genome editing via microhomology‐mediated end joining
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DOI: 10.1111/pbi.13490
Abstract: Summary Gene editing and/or allele introgression with absolute precision and control appear to be the ultimate goals of genetic engineering. Precision genome editing in plants has been developed through various approaches, including oligonucleotide‐directed mutagenesis (ODM),… read more here.
Keywords: genome editing; microhomology mediated; crispr cas; precision genome ... See more keywords
Quantifying the mapping precision of genome-wide association studies using whole-genome sequencing data
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DOI: 10.1186/s13059-017-1216-0
Abstract: BackgroundUnderstanding the mapping precision of genome-wide association studies (GWAS), that is the physical distances between the top associated single-nucleotide polymorphisms (SNPs) and the causal variants, is essential to design fine-mapping experiments for complex traits and… read more here.
Keywords: causal variants; genome wide; mapping precision; association ... See more keywords