Genomic distribution of 5‐formylcytosine and 5‐carboxylcytosine in human preimplantation embryos
Sign Up to like & getrecommendations! Published in 2018 at "Molecular Reproduction and Development"
DOI: 10.1002/mrd.23074
Abstract: 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) are derivatives of 5-methylcytosine (5mC). Sequential enzymatic oxidation of 5mC produces 5-hydroxymethylcytosine (5hmC), 5fC, and 5caC. Similarly to 5hmC, 5fC and 5caC serve as transient intermediates in active DNA demethylation… read more here.
Keywords: formylcytosine; carboxylcytosine; preimplantation embryos; genomic distribution ... See more keywords
Immunochemical Detection of Modified Cytosine Species in Mammalian Preimplantation Embryos.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0876-0_12
Abstract: DNA methylation undergoes dynamic changes at the genome-wide scale during the early steps of mammalian embryo development. Immunochemical detection of 5-methylcytosine (5mC) in the zygote has led to the discovery that a global loss of… read more here.
Keywords: cytosine species; dna; preimplantation embryos; immunochemical detection ... See more keywords
Low-dose Roundup induces developmental toxicity in bovine preimplantation embryos in vitro
Sign Up to like & getrecommendations! Published in 2020 at "Environmental Science and Pollution Research"
DOI: 10.1007/s11356-020-08183-8
Abstract: Roundup is a widely used glyphosate-based herbicide worldwide. Roundup residues can be detected in the organs and urine of animals. However, its toxicity on mammalian preimplantation embryos has not been well investigated. Here, we show… read more here.
Keywords: roundup; preimplantation embryos; preimplantation; toxicity ... See more keywords
Glucocorticoid receptor isoforms and effects of glucocorticoids in ovulated mouse oocytes and preimplantation embryos†
Sign Up to like & getrecommendations! Published in 2018 at "Biology of Reproduction"
DOI: 10.1093/biolre/ioy196
Abstract: ABSTRACT To investigate possible involvement of glucocorticoid receptor (GR) in mediating effects of maternal stress or therapeutically administered glucocorticoids on early embryo, we analyzed the expression of GR subtypes in ovulated mouse oocytes and preimplantation… read more here.
Keywords: ovulated mouse; preimplantation embryos; mouse oocytes; glucocorticoid ... See more keywords
P–137 Male embryos take longer to develop to the blastocysts stage
Sign Up to like & getrecommendations! Published in 2021 at "Human Reproduction"
DOI: 10.1093/humrep/deab130.136
Abstract: Are there any differences in developmental timings between male and female preimplantation embryos? There is a tendency for statistical difference in the time to reach blastocyst stage for male embryos compared to female embryos Differences… read more here.
Keywords: embryos; preimplantation embryos; time; male embryos ... See more keywords
The incidence and origin of segmental aneuploidy in human oocytes and preimplantation embryos
Sign Up to like & getrecommendations! Published in 2017 at "Human Reproduction"
DOI: 10.1093/humrep/dex324
Abstract: STUDY QUESTION What is the incidence, origin and clinical significance of segmental aneuploidy in human oocytes and preimplantation embryos? SUMMARY ANSWER Segmental aneuploidy occurs at a considerable frequency in preimplantation embryos with a majority being… read more here.
Keywords: origin; preimplantation embryos; aneuploidy; segmental aneuploidy ... See more keywords
Single-cell sequencing of primate preimplantation embryos reveals chromosome elimination via cellular fragmentation and blastomere exclusion.
Sign Up to like & getrecommendations! Published in 2019 at "Genome research"
DOI: 10.1101/gr.239830.118
Abstract: Aneuploidy that arises during meiosis and/or mitosis is a major contributor to early embryo loss. We previously showed that human preimplantation embryos encapsulate missegregated chromosomes into micronuclei while undergoing cellular fragmentation and that fragments can… read more here.
Keywords: single cell; cellular fragmentation; preimplantation embryos; cell ... See more keywords
DNA methylation changes during preimplantation development reveal inter-species differences and reprogramming events at imprinted genes
Sign Up to like & getrecommendations! Published in 2020 at "Clinical Epigenetics"
DOI: 10.1186/s13148-020-00857-x
Abstract: Preimplantation embryos experience profound resetting of epigenetic information inherited from the gametes. Genome-wide analysis at single-base resolution has shown similarities but also species differences between human and mouse preimplantation embryos in DNA methylation patterns and… read more here.
Keywords: methylation; species differences; imprinted genes; preimplantation embryos ... See more keywords
5-Formylcytosine landscapes of human preimplantation embryos at single-cell resolution
Sign Up to like & getrecommendations! Published in 2020 at "PLoS Biology"
DOI: 10.1371/journal.pbio.3000799
Abstract: Epigenetic dynamics, such as DNA methylation and chromatin accessibility, have been extensively explored in human preimplantation embryos. However, the active demethylation process during this crucial period remains largely unexplored. In this study, we use single-cell… read more here.
Keywords: single cell; preimplantation embryos; human preimplantation;
Direct Injection of Recombinant AAV-Containing Solution into the Oviductal Lumen of Pregnant Mice Caused In Situ Infection of Both Preimplantation Embryos and Oviductal Epithelium
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23094897
Abstract: Adeno-associated virus (AAV) vector is an efficient viral-based gene delivery tool used with many types of cells and tissues, including neuronal cells and muscles. AAV serotype 6 (AAV-6), one of numerous AAV serotypes, was recently… read more here.
Keywords: aav; oviductal lumen; recombinant aav; preimplantation embryos ... See more keywords
The cytotoxic effects of dimethyl sulfoxide in mouse preimplantation embryos: a mechanistic study
Sign Up to like & getrecommendations! Published in 2017 at "Theranostics"
DOI: 10.7150/thno.21662
Abstract: Rationale: Dimethyl sulfoxide (DMSO) is commonly used as a solvent for water-insoluble substances, a vehicle for drug therapy, and a cryoprotectant for cultured cells. DMSO induced embryonic defects and its mechanism of action remains unclear.… read more here.
Keywords: cytotoxic effects; cell; preimplantation embryos; dmso induced ... See more keywords