Hapten-Anti-Hapten Technique for Two-Color IHC Detection of Phosphorylated EGFR and H2AX Using Primary Antibodies Raised in the Same Host Species.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6759-9_8
Abstract: Multiplex staining of cell and tissue sections with antibodies raised in the same host species is a serious challenge because of unwanted but inevitable cross-reactivity of secondary antibodies with irrelevant primary antibodies. Several techniques can… read more here.
Keywords: host species; raised host; two color; hapten anti ... See more keywords
Efficient Small-Scale Conjugation of DNA to Primary Antibodies for Multiplexed Cellular Targeting
Sign Up to like & getrecommendations! Published in 2019 at "Bioconjugate Chemistry"
DOI: 10.1021/acs.bioconjchem.9b00490
Abstract: The combination of the specificity of antibodies and the programmability of DNA nanotechnology has provided the scientific community with a powerful tool to label and unambiguously distinguish a large number of subcellular targets using fluorescence-based… read more here.
Keywords: dna; efficient small; scale conjugation; small scale ... See more keywords