High-efficiency RNA-based reprogramming of human primary fibroblasts
Sign Up to like & getrecommendations! Published in 2018 at "Nature Communications"
DOI: 10.1038/s41467-018-03190-3
Abstract: Induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine; however, their potential clinical application is hampered by the low efficiency of somatic cell reprogramming. Here, we show that the synergistic activity of synthetic… read more here.
Keywords: medicine; primary fibroblasts; synergistic activity; reprogramming human ... See more keywords
Combinational use of lipid-based reagents for efficient transfection of primary fibroblasts and hepatoblasts.
Sign Up to like & getrecommendations! Published in 2017 at "BioTechniques"
DOI: 10.2144/000114569
Abstract: Commercially available lipid-based transfection reagents are widely used to deliver DNA to cells. However, these lipid-based transfection reagents show poor gene transfer efficiency in primary cells. Here, we demonstrate a simple method to improve gene… read more here.
Keywords: transfection; primary fibroblasts; transfection reagents; lipid based ... See more keywords
Perturbed actin cap as a new personalized biomarker in primary fibroblasts of Huntington’s disease patients
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Cell and Developmental Biology"
DOI: 10.3389/fcell.2023.1013721
Abstract: Primary fibroblasts from patient’s skin biopsies are directly isolated without any alteration in the genome, retaining in culture conditions their endogenous cellular characteristics and biochemical properties. The aim of this study was to identify a… read more here.
Keywords: disease patients; huntington disease; primary fibroblasts; cap ... See more keywords
Metformin Treatment Induces Different Response in Pheochromocytoma/Paraganglioma Tumour Cells and in Primary Fibroblasts
Sign Up to like & getrecommendations! Published in 2022 at "Cancers"
DOI: 10.3390/cancers14143471
Abstract: Simple Summary Pheochromocytoma/paragangliomas (PPGLs) are neuroendocrine tumours and are often non-metastatic. However, no effective treatment is available for their metastatic form. Recent studies have shown that metformin exhibits antiproliferative activity in many human cancers, including… read more here.
Keywords: tumour microenvironment; tumour cells; primary fibroblasts; pheochromocytoma ... See more keywords
Cell Behavior of Primary Fibroblasts and Osteoblasts on Plasma-Treated Fluorinated Polymer Coated with Honeycomb Polystyrene
Sign Up to like & getrecommendations! Published in 2021 at "Materials"
DOI: 10.3390/ma14040889
Abstract: The development of new biocompatible polymer substrates is still of interest to many research teams. We aimed to combine a plasma treatment of fluorinated ethylene propylene (FEP) substrate with a technique of improved phase separation.… read more here.
Keywords: primary fibroblasts; chemistry; polymer; fibroblasts osteoblasts ... See more keywords
Bovine Papillomavirus Type 1 or 2 Virion-Infected Primary Fibroblasts Constitute a Near-Natural Equine Sarcoid Model
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DOI: 10.3390/v14122658
Abstract: Equine sarcoids are common, locally aggressive skin tumors induced by bovine papillomavirus types 1, 2, and possibly 13 (BPV1, BPV2, BPV13). Current in vitro models do not mimic de novo infection. We established primary fibroblasts… read more here.
Keywords: equine sarcoid; dna; bovine papillomavirus; primary fibroblasts ... See more keywords