Application of a cost-effective DNA extraction protocol for screening transgenic and CRISPR-edited primary goat cells
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DOI: 10.1371/journal.pone.0239435
Abstract: The genotyping of genetically-modified cells is a crucial step in studies of transgenics and genomic editing with systems such as CRISPR/Cas. The detection of genome editing events can be directly related to the genotyping methodology… read more here.
Keywords: methodology; extraction; primary goat; protocol ... See more keywords
CRISPR/Cas9-Induced Knockout of miR-24 Reduces Cholesterol and Monounsaturated Fatty Acid Content in Primary Goat Mammary Epithelial Cells
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DOI: 10.3390/foods11142012
Abstract: In nonruminants, microRNA (miRNA)-24 plays an important role in lipid metabolism in adipose tissue and the liver. Although the abundance of miR-24 in ruminant mammary glands is the highest during peak lactation, its potential role… read more here.
Keywords: crispr cas9; primary goat; cholesterol; goat mammary ... See more keywords