Quantitative Proteomic Analysis of Simian Primary Hepatocytes Reveals Candidate Molecular Markers for Permissiveness to Relapsing Malaria Plasmodium cynomolgi
Sign Up to like & getrecommendations! Published in 2019 at "PROTEOMICS"
DOI: 10.1002/pmic.201900021
Abstract: A major obstacle impeding malaria research is the lack of an in vitro system capable of supporting infection through the entire liver stage cycle of the parasite, including that of the dormant forms known as… read more here.
Keywords: quantitative proteomic; primary hepatocytes; infection; liver stage ... See more keywords
Alterations in cellular lipid metabolism produce neutral lipid accumulation following exposure to the organochlorine compound trans‐nonachlor in rat primary hepatocytes
Sign Up to like & getrecommendations! Published in 2018 at "Environmental Toxicology"
DOI: 10.1002/tox.22583
Abstract: Recent epidemiological studies have revealed significant positive associations between exposure to organochlorine (OC) pesticides and occurrence of the metabolic syndrome and there are a growing number of animal‐based studies to support causality. However, the cellular… read more here.
Keywords: primary hepatocytes; fatty acid; exposure; exposure organochlorine ... See more keywords
Isolation of Primary Hepatocytes for Testing Tumor Penetrating Peptides.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1752-6_26
Abstract: Methods for isolation of human primary cells is an important tool to be able to test the effect of several drugs for the treatment of diseases. Protocols have been described for the isolation of healthy… read more here.
Keywords: primary hepatocytes; penetrating peptides; tumor penetrating; isolation primary ... See more keywords
Nimbolide attenuate the lipid accumulation, oxidative stress and antioxidant in primary hepatocytes
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Biology Reports"
DOI: 10.1007/s11033-017-4132-1
Abstract: Nimbolide is a bioactive compound found in Azadirachta indica. This work was devised to investigate the potential effects of nimbolide on intracellular lipid deposition and its associated redox modulation in primary hepatocytes (Heps). Lipid accumulation… read more here.
Keywords: accumulation; nimbolide may; primary hepatocytes; lipid accumulation ... See more keywords
Disruption of classical estrogenic targets in brown trout primary hepatocytes by the model androgens testosterone and dihydrotestosterone.
Sign Up to like & getrecommendations! Published in 2020 at "Aquatic toxicology"
DOI: 10.1016/j.aquatox.2020.105586
Abstract: Estrogenic effects triggered by androgens have been previously shown in a few studies. Aromatization and direct binding to estrogen receptors (ERs) are the most proposed mechanisms. For example, previously, a modulation of vitellogenin A (VtgA)… read more here.
Keywords: estrogenic targets; disruption; dht 100; primary hepatocytes ... See more keywords
Cytotoxicity of three graphene-related materials in rainbow trout primary hepatocytes is not associated to cellular internalization.
Sign Up to like & getrecommendations! Published in 2022 at "Ecotoxicology and environmental safety"
DOI: 10.1016/j.ecoenv.2022.113227
Abstract: As a consequence of increasing production and use of graphene-related materials (GRM), their release into the aquatic environment is likely to be expected. Development of appropriate model systems to assess their potential toxicity toward aquatic… read more here.
Keywords: rainbow trout; graphene related; related materials; cytotoxicity ... See more keywords
Controlled cell morphology and liver-specific function of engineered primary hepatocytes by fibroblast layer cell densities.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of bioscience and bioengineering"
DOI: 10.1016/j.jbiosc.2018.02.006
Abstract: Engineered primary hepatocytes, including co-cultured hepatocyte sheets, are an attractive to basic scientific and clinical researchers because they maintain liver-specific functions, have reconstructed cell polarity, and have high transplantation efficiency. However, co-culture conditions regarding engineered… read more here.
Keywords: primary hepatocytes; tig 118; liver specific; morphology liver ... See more keywords
Fabrication of liver-derived extracellular matrix nanofibers and functional evaluation in in vitro culture using primary hepatocytes
Sign Up to like & getrecommendations! Published in 2018 at "Materialia"
DOI: 10.1016/j.mtla.2018.11.014
Abstract: Abstract Fabrication of functional scaffolds is one of the main goals of tissue engineering, particularly scaffolds mimicking the native extracellular matrix (ECM) and stimulating a microenvironment that supports cell growth and function. In this study,… read more here.
Keywords: extracellular matrix; liver derived; vitro culture; primary hepatocytes ... See more keywords
Genotoxicity evaluation using primary hepatocytes isolated from rhesus macaque (Macaca mulatta).
Sign Up to like & getrecommendations! Published in 2021 at "Toxicology"
DOI: 10.1016/j.tox.2021.152936
Abstract: Non-human primates (NHPs) have played a vital role in fundamental, pre-clinical, and translational studies because of their high physiological and genetic similarity to humans. Here, we report a method to isolate primary hepatocytes from the… read more here.
Keywords: macaca mulatta; primary hepatocytes; genotoxicity; macaque ... See more keywords
The viability of primary hepatocytes is maintained under a low cysteine‐glutathione redox state with a marked elevation in ophthalmic acid production
Sign Up to like & getrecommendations! Published in 2017 at "Experimental Cell Research"
DOI: 10.1016/j.yexcr.2017.10.017
Abstract: Abstract Extracellular cystine, the oxidized form of cysteine (Cys), is taken up by cells via the cystine transporter xCT. xCT is not expressed in the liver but is induced in primary hepatocytes under conventional cultured… read more here.
Keywords: ophthalmic acid; primary hepatocytes; cysteine; gsh ... See more keywords
Biochemical and anaplerotic applications of in vitro models of propionic acidemia and methylmalonic acidemia using patient-derived primary hepatocytes.
Sign Up to like & getrecommendations! Published in 2020 at "Molecular genetics and metabolism"
DOI: 10.1016/j.ymgme.2020.05.003
Abstract: Propionic acidemia (PA) and methylmalonic acidemia (MMA) are autosomal recessive disorders of propionyl-CoA (P-CoA) catabolism, which are caused by a deficiency in the enzyme propionyl-CoA carboxylase or the enzyme methylmalonyl-CoA (MM-CoA) mutase, respectively. The functional… read more here.
Keywords: primary hepatocytes; coa coa; coa; tca cycle ... See more keywords