Decellularized Liver Nanofibers Enhance and Stabilize the Long-term Functions of Primary Human Hepatocytes In Vitro.
Sign Up to like & getrecommendations! Published in 2023 at "Advanced healthcare materials"
DOI: 10.1002/adhm.202202302
Abstract: Owing to significant differences across species in liver functions, in vitro human liver models are used for screening the metabolism and toxicity of compounds, modeling diseases, and cell-based therapies. However, the extracellular matrix (ECM) scaffold… read more here.
Keywords: human hepatocytes; liver; primary human; topography ... See more keywords
Age‐specific effectiveness of primary human papillomavirus screening versus cytology in a cervical cancer screening program: a nationwide cross‐sectional study
Sign Up to like & getrecommendations! Published in 2022 at "Cancer Communications"
DOI: 10.1002/cac2.12256
Abstract: Primary human papillomavirus (HPV) screening is recommended for the detection of cervical intraepithelial neoplasia (CIN) in the general population; however, the triage for HPV‐positive women remains a challenge. This study aimed to evaluate the age‐specific… read more here.
Keywords: specific effectiveness; primary human; age specific; human papillomavirus ... See more keywords
IgE‐mediated regulation of IL‐10 and type I IFN enhances rhinovirus‐induced Th2 differentiation by primary human monocytes
Sign Up to like & getrecommendations! Published in 2020 at "European Journal of Immunology"
DOI: 10.1002/eji.201948396
Abstract: Rhinovirus (RV) infections are linked to the development and exacerbation of allergic diseases including allergic asthma. IgE, another contributor to atopic disease pathogenesis, has been shown to regulate DC antiviral functions and influence T cell… read more here.
Keywords: ige mediated; th2 differentiation; induced th2; rhinovirus ... See more keywords
Harpagoside suppresses IL‐6 expression in primary human osteoarthritis chondrocytes
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Orthopaedic Research"
DOI: 10.1002/jor.23262
Abstract: There is growing evidence in support of the involvement of inflammatory response in the pathogenesis of osteoarthritis (OA). Harpagoside, one of the bioactive components of Harpagophytum procumbens (Hp), has been shown to possess anti‐inflammatory properties.… read more here.
Keywords: suppresses expression; harpagoside suppresses; osteoarthritis; chondrocytes harpagoside ... See more keywords
Cultivation of Encapsulated Primary Human B Lymphocytes: A First Step towards a Bioartificial Germinal Center.
Sign Up to like & getrecommendations! Published in 2022 at "Macromolecular bioscience"
DOI: 10.1002/mabi.202200256
Abstract: Polyelectrolyte microcapsules based on sodium cellulose sulfate (SCS) and poly-diallyl-dimethyl-ammonium chloride (PDADMAC) have previously been proposed as a suitable ex vivo microenvironment for the cultivation and differentiation of primary human T lymphocytes. Here, the same… read more here.
Keywords: cell; primary human; cultivation; human lymphocytes ... See more keywords
2D Materials and Primary Human Dendritic Cells: A Comparative Cytotoxicity Study.
Sign Up to like & getrecommendations! Published in 2022 at "Small"
DOI: 10.1002/smll.202107652
Abstract: Human health can be affected by materials indirectly through exposure to the environment or directly through close contact and uptake. With the ever-growing use of 2D materials in many applications such as electronics, medical therapeutics,… read more here.
Keywords: primary human; materials primary; human dendritic; cells comparative ... See more keywords
CRISPR/Cas9-Mediated Genome Engineering of Primary Human B Cells.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0290-4_24
Abstract: The CRISPR/Cas9 system allows for site-specific gene editing and genome engineering of primary human cells. Here we describe methods for gene editing and genome engineering of B cells isolated from human peripheral blood mononuclear cells… read more here.
Keywords: primary human; crispr cas9; engineering primary; genome engineering ... See more keywords
Efficient RNA-Based Reprogramming of Disease-Associated Primary Human Fibroblasts into Induced Pluripotent Stem Cells.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0301-7_17
Abstract: Reprogramming a patient's somatic cells into induced pluripotent stem cells (iPSCs) holds great promise for disease modeling and the development of autologous cellular therapeutics. However, it remains challenging to consistently reprogram primary human cells, as… read more here.
Keywords: disease; rna based; pluripotent stem; induced pluripotent ... See more keywords
An Organotypic 3D Assay for Primary Human Mammary Epithelial Cells that Recapitulates Branching Morphogenesis.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7021-6_9
Abstract: We have developed a three-dimensional organotypic culture system for primary human mammary epithelial cells (HMECs) in which the cells are cultured in free floating collagen type I gels. In this assay, luminal cells predominantly form… read more here.
Keywords: epithelial cells; mammary epithelial; branching morphogenesis; human mammary ... See more keywords
Transcriptomic Analysis of Cholestatic Compounds In Vitro.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9420-5_12
Abstract: Drug-induced cholestasis is one of the most severe manifestations of drug-induced liver injury. Drug-induced cholestasis is characterized by an accumulation of endogenous metabolites normally excreted in the bile such as bile salts, cholesterol, bilirubin, or… read more here.
Keywords: drug induced; drug; induced cholestasis; primary human ... See more keywords
Establishment of Sandwich Cultures of Primary Human Hepatocytes.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9420-5_21
Abstract: Primary hepatocytes and their adherent cultures are still considered as the golden standard in the field of liver-based in vitro modeling. However, they cope with progressive deterioration of their in vivo-like morphological and functional phenotype.… read more here.
Keywords: cultures primary; establishment sandwich; biology; sandwich cultures ... See more keywords