Tracking Tau in Neurons: How to Grow, Fix, and Stain Primary Neurons for the Investigation of Tau in All Developmental Stages.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6598-4_20
Abstract: Primary neurons have proved to be an invaluable tool for the investigation of Tau in the context of neuronal development and neurodegeneration. Culturing neurons usually is time consuming and requires multiple feeding steps and media… read more here.
Keywords: tracking tau; tau neurons; primary neurons; investigation tau ... See more keywords
Tracking Tau in Neurons: How to Transfect and Track Exogenous Tau into Primary Neurons.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6598-4_21
Abstract: Primary neurons have proved to be an essential tool for investigating neuronal polarity in general and polarized Tau distribution in particular. However, mature primary neurons are notoriously difficult to transfect with nonviral vectors and are… read more here.
Keywords: tracking tau; tau; primary neurons; tau neurons ... See more keywords
Mechanism of miR-320 in Regulating Biological Characteristics of Ischemic Cerebral Neuron by Mediating Nox2/ROS Pathway
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Molecular Neuroscience"
DOI: 10.1007/s12031-019-01434-5
Abstract: This study aimed to explore the mechanism of miR-320 in regulating biological characteristics of ischemic cerebral neuron by mediating Nox2/ROS pathway. Primary neurons were cultured and grouped: normal group (normal primary neurons), negative control (NC)… read more here.
Keywords: nox2 ros; primary neurons; group; ischemic cerebral ... See more keywords
Metabolic Glycan Labeling in Primary Neurons Enabled by Unnatural Sugars with No S-Glyco-Modification.
Sign Up to like & getrecommendations! Published in 2023 at "ACS chemical biology"
DOI: 10.1021/acschembio.3c00152
Abstract: It is of great interest to probe glycosylation in primary neuron cultures. However, per-O-acetylated clickable unnatural sugars, which have been routinely utilized in metabolic glycan labeling (MGL) for analyzing glycans, showed cytotoxicity to cultured primary… read more here.
Keywords: metabolic glycan; primary neurons; unnatural sugars; glyco modification ... See more keywords
MiR‐132 down‐regulates high glucose‐induced β‐dystroglycan degradation through Matrix Metalloproteinases‐9 up‐regulation in primary neurons
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Cellular and Molecular Medicine"
DOI: 10.1111/jcmm.16669
Abstract: Cognitive dysfunction is one of the complications of diabetes. Unfortunately, there is no effective methods to block its progression currently. One of the pathophysiological mechanisms is synaptic protein damage and neuronal signal disruption because of… read more here.
Keywords: protein; degradation; mir 132; primary neurons ... See more keywords
Nicotinamide attenuates the decrease in dendritic spine density in hippocampal primary neurons from 5xFAD mice, an Alzheimer’s disease animal model
Sign Up to like & getrecommendations! Published in 2020 at "Molecular Brain"
DOI: 10.1186/s13041-020-0565-x
Abstract: Alzheimer’s disease (AD) is the most common neurodegenerative disease characterized by memory loss and the presence of amyloid plaques and neurofibrillary tangles in the patients’ brains. In this study, we investigated the alterations in metabolite… read more here.
Keywords: primary neurons; spine density; hippocampal primary; disease ... See more keywords
Recent progresses in novel in vitro models of primary neurons: A biomaterial perspective
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.953031
Abstract: Central nervous system (CNS) diseases have been a growing threat to the health of humanity, emphasizing the urgent need of exploring the pathogenesis and therapeutic approaches of various CNS diseases. Primary neurons are directly obtained… read more here.
Keywords: culture; vitro models; primary neurons; novel vitro ... See more keywords