Employing QbD strategies to assess the impact of cell viability and density on the primary recovery of monoclonal antibodies
Sign Up to like & getrecommendations! Published in 2022 at "Engineering in Life Sciences"
DOI: 10.1002/elsc.202200056
Abstract: Quality by Design (QbD) is one of the most important tools for the implementation of Process Analytical Technology (PAT) in biopharmaceutical production. For optimal characterization of a monoclonal antibody (mAb) upstream process a stepwise approach… read more here.
Keywords: primary recovery; cell; process; cell viability ... See more keywords
pH conditioning is a crucial step in primary recovery - A case study for a recombinant Fab from E. coli.
Sign Up to like & getrecommendations! Published in 2019 at "Protein expression and purification"
DOI: 10.1016/j.pep.2019.105504
Abstract: Primary recovery of recombinant proteins from E. coli often describes a major challenge in downstream processing. After product release, the target protein usually accounts for only a small amount of total protein and has to… read more here.
Keywords: recovery; recombinant fab; primary recovery; step ... See more keywords
Primary recovery of hyaluronic acid produced in Streptococcus equi subsp. zooepidemicus using PEG–citrate aqueous two-phase systems
Sign Up to like & getrecommendations! Published in 2021 at "AMB Express"
DOI: 10.1186/s13568-021-01287-5
Abstract: Given its biocompatibility, rheological, and physiological properties, hyaluronic acid (HA) has become a biomaterial of increasing interest with multiple applications in medicine and cosmetics. In recent decades, microbial fermentations have become an important source for… read more here.
Keywords: recovery; phase; primary recovery; peg citrate ... See more keywords