Modification of the 4Fe-4S Cluster Charge Transport Pathway Alters RNA Synthesis by Yeast DNA Primase.
Sign Up to like & getrecommendations! Published in 2022 at "Biochemistry"
DOI: 10.1021/acs.biochem.2c00100
Abstract: DNA synthesis during replication begins with the generation of an ∼10-nucleotide primer by DNA primase. Primase contains a redox-active 4Fe-4S cluster in the C-terminal domain of the p58 subunit (p58C). The redox state of this… read more here.
Keywords: 4fe cluster; primase; dna; charge transport ... See more keywords
Identification of a Ligand-Binding Site on the Staphylococcus aureus DnaG Primase C-Terminal Domain.
Sign Up to like & getrecommendations! Published in 2017 at "Biochemistry"
DOI: 10.1021/acs.biochem.6b01273
Abstract: The interface between the DnaG primase C-terminal domain (CTD) and the N-terminal domain of DnaB helicase is essential for bacterial DNA replication because it allows coordinated priming of DNA synthesis at the replication fork while… read more here.
Keywords: terminal domain; ctd; primase; conformation ... See more keywords
Theoretical Development of DnaG Primase as a Novel Narrow-Spectrum Antibiotic Target
Sign Up to like & getrecommendations! Published in 2022 at "ACS Omega"
DOI: 10.1021/acsomega.1c05928
Abstract: The widespread use of antibiotics to treat infections is one of the reasons that global mortality rates have fallen over the past 80 years. However, antibiotic use is also responsible for the concomitant rise in… read more here.
Keywords: theoretical development; primase novel; development dnag; novel narrow ... See more keywords
The combined DNA and RNA synthetic capabilities of archaeal DNA primase facilitate primer hand-off to the replicative DNA polymerase
Sign Up to like & getrecommendations! Published in 2022 at "Nature Communications"
DOI: 10.1038/s41467-022-28093-2
Abstract: Replicative DNA polymerases cannot initiate DNA synthesis de novo and rely on dedicated RNA polymerases, primases, to generate a short primer. This primer is then extended by the DNA polymerase. In diverse archaeal species, the… read more here.
Keywords: dna; dna polymerase; primer; primase ... See more keywords
Primase promotes the competition between transcription and replication on the same template strand resulting in DNA damage
Sign Up to like & getrecommendations! Published in 2024 at "Nature Communications"
DOI: 10.1038/s41467-023-44443-0
Abstract: Resolving R-loops caused by transcription-replication conflicts (TRCs) is vital to genome stability in organisms. Here, the authors show that the chloroplast-localized primase ATH intensifies template strand competition and exacerbates the Head-On TRCs induced DNA damage.… read more here.
Keywords: dna damage; transcription; transcription replication; primase ... See more keywords
Identification of potential inhibitor against Leishmania donovani mitochondrial DNA primase through in-silico and in vitro drug repurposing approaches
Sign Up to like & getrecommendations! Published in 2024 at "Scientific Reports"
DOI: 10.1038/s41598-024-53316-5
Abstract: Leishmania donovani is the causal organism of leishmaniasis with critical health implications affecting about 12 million people around the globe. Due to less efficacy, adverse side effects, and resistance, the available therapeutic molecules fail to… read more here.
Keywords: leishmania; drug; leishmania donovani; primase ... See more keywords
Helicase-primase inhibitors from Medshine Discovery Inc. (WO2018/127207 and WO2020/007355) for the treatment of herpes simplex virus infections – structure proposal for Phaeno Therapeutics drug candidate HN0037
Sign Up to like & getrecommendations! Published in 2022 at "Expert Opinion on Therapeutic Patents"
DOI: 10.1080/13543776.2022.2113873
Abstract: ABSTRACT Helicase-primase is an interesting target for small-molecule therapy of herpes simplex virus (HSV) infections. With amenamevir already approved for varicella-zoster virus and with pritelivir’s granted breakthrough therapy designation for the treatment of acyclovir-resistant HSV… read more here.
Keywords: simplex virus; drug candidate; phaeno therapeutics; helicase primase ... See more keywords
Inferring primase-DNA specific recognition using a data driven approach
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkab956
Abstract: Abstract DNA–protein interactions play essential roles in all living cells. Understanding of how features embedded in the DNA sequence affect specific interactions with proteins is both challenging and important, since it may contribute to finding… read more here.
Keywords: protein interactions; dna; inferring primase; dna protein ... See more keywords
The monomeric archaeal primase from Nanoarchaeum equitans harbours the features of heterodimeric archaeoeukaryotic primases and primes sequence-specifically.
Sign Up to like & getrecommendations! Published in 2023 at "Nucleic acids research"
DOI: 10.1093/nar/gkad261
Abstract: Abstract The marine thermophilic archaeon Nanoarchaeum equitans possesses a monomeric primase encompassing the conserved domains of the small catalytic and the large regulatory subunits of archaeoeukaryotic heterodimeric primases in one protein chain. The recombinant protein… read more here.
Keywords: monomeric archaeal; nanoarchaeum equitans; heterodimeric archaeoeukaryotic; archaeoeukaryotic primases ... See more keywords
A four-in-one replicase integrating key enzymatic activities for DNA replication
Sign Up to like & getrecommendations! Published in 2025 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkaf542
Abstract: Abstract DNA replication is a fundamental process in all living organisms. As the most diverse and abundant biological entities on Earth, bacteriophages may utilize unconventional methods for genome replication. In this study, we identified a… read more here.
Keywords: replication; dna replication; primase; replicase ... See more keywords
Response to Comments on “The [4Fe4S] cluster of human DNA primase functions as a redox switch using DNA charge transport”
Sign Up to like & getrecommendations! Published in 2017 at "Science"
DOI: 10.1126/science.aan2762
Abstract: Baranovskiy et al. and Pellegrini argue that, based on structural data, the path for charge transfer through the [4Fe4S] domain of primase is not feasible. Our manuscript presents electrochemical data directly showing charge transport through… read more here.
Keywords: charge transport; dna; charge; primase ... See more keywords