Enhancing Prime Editing Efficiency Through Modulation of Methylation on the Newly Synthesized DNA Strand and Prolonged Expression
Sign Up to like & getrecommendations! Published in 2025 at "Advanced Science"
DOI: 10.1002/advs.202417790
Abstract: Prime editors (PEs) have emerged as transformative tools for precision genome engineering, yet their broader application remains constrained by incomplete understanding of repair mechanisms. In this study, it is found that an increase in the… read more here.
Keywords: editing efficiency; methylation; efficiency; prime editing ... See more keywords
Prime editing in plants and mammalian cells: Mechanism, achievements, limitations, and future prospects
Sign Up to like & getrecommendations! Published in 2022 at "BioEssays"
DOI: 10.1002/bies.202200032
Abstract: Clustered, regularly interspaced, short palindromic repeat (CRISPR)/CRISPR‐associated protein (CRISPR/Cas) system has revolutionized genetic research in the life sciences. Four classes of CRISPR/Cas‐derived genome editing agents, such as nuclease, base editor, recombinase, and prime editor have… read more here.
Keywords: genome editing; crispr; prime editing; plants mammalian ... See more keywords
Adenine base editing and prime editing of chemically derived hepatic progenitors rescue genetic liver disease.
Sign Up to like & getrecommendations! Published in 2021 at "Cell stem cell"
DOI: 10.1016/j.stem.2021.04.010
Abstract: DNA base editors and prime editing technology enable therapeutic in situ correction of disease-causing alleles. These techniques could have broad applications for ex vivo editing of cells prior to transplantation in a range of diseases, but… read more here.
Keywords: derived hepatic; hepatic progenitors; disease; base ... See more keywords
Prime Editing of TaEPSPS Homeologs Enhances Wheat Resistance to Glyphosate.
Sign Up to like & getrecommendations! Published in 2025 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.5c05769
Abstract: 5-Enolpyruvylshikimate-3-phosphate synthase (EPSPS) is the primary target of the broad-spectrum herbicide glyphosate, and several well-characterized amino acid substitutions in EPSPS are known to confer glyphosate resistance. Here, we introduced the P106S substitution into wheat TaEPSPS… read more here.
Keywords: resistance; wheat; p106s; prime editing ... See more keywords
Prime Editing Guide RNA Design Automation Using PINE-CONE
Sign Up to like & getrecommendations! Published in 2021 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.0c00445
Abstract: CRISPR-based technologies are paramount in genome engineering and synthetic biology. Prime editing (PE) is a technology capable of installing genomic edits without double-stranded DNA breaks (DSBs) or donor DNA. Prime editing guide RNAs (pegRNAs) simultaneously… read more here.
Keywords: pine cone; guide; biology; prime editing ... See more keywords
WT-PE: Prime editing with nuclease wild-type Cas9 enables versatile large-scale genome editing
Sign Up to like & getrecommendations! Published in 2022 at "Signal Transduction and Targeted Therapy"
DOI: 10.1038/s41392-022-00936-w
Abstract: Large scale genomic aberrations including duplication, deletion, translocation, and other structural changes are the cause of a subtype of hereditary genetic disorders and contribute to onset or progress of cancer. The current prime editor, PE2,… read more here.
Keywords: large scale; prime editing; type cas9; nuclease wild ... See more keywords
Efficient generation of mouse models with the prime editing system
Sign Up to like & getrecommendations! Published in 2020 at "Cell Discovery"
DOI: 10.1038/s41421-020-0165-z
Abstract: Dear Editor, Most genetic diseases in humans are caused by singlenucleotide mutations. Although genome editing with either the CRISPR-based cytosine base editor (CBE) or the adenine base editor (ABE) holds great promise for gene correction… read more here.
Keywords: system; base; prime editing; efficiency ... See more keywords
PrimeDesign software for rapid and simplified design of prime editing guide RNAs.
Sign Up to like & getrecommendations! Published in 2021 at "Nature communications"
DOI: 10.1038/s41467-021-21337-7
Abstract: Prime editing (PE) is a versatile genome editing technology, but design of the required guide RNAs is more complex than for standard CRISPR-based nucleases or base editors. Here we describe PrimeDesign, a user-friendly, end-to-end web… read more here.
Keywords: prime editing; guide rnas; editing guide; design ... See more keywords
Robust and inducible genome editing via an all-in-one prime editor in human pluripotent stem cells
Sign Up to like & getrecommendations! Published in 2024 at "Nature Communications"
DOI: 10.1038/s41467-024-55104-1
Abstract: Prime editing (PE) allows for precise genome editing in human pluripotent stem cells (hPSCs), such as introducing single nucleotide modifications, small insertions or deletions at a specific genomic locus. Here, we systematically compare a panel… read more here.
Keywords: pluripotent stem; human pluripotent; stem cells; prime editing ... See more keywords
Delivery of Prime editing in human stem cells using pseudoviral NanoScribes particles
Sign Up to like & getrecommendations! Published in 2025 at "Nature Communications"
DOI: 10.1038/s41467-024-55604-0
Abstract: Prime Editing can rewrite genes in living cells by allowing point mutations, deletions, or insertion of small DNA sequences with high precision. However, its safe and efficient delivery into human stem cells remains a technical… read more here.
Keywords: prime editing; delivery prime; human stem; stem cells ... See more keywords
Prime editor with rational design and AI-driven optimization for reverse editing window and enhanced fidelity
Sign Up to like & getrecommendations! Published in 2025 at "Nature Communications"
DOI: 10.1038/s41467-025-60495-w
Abstract: Prime editing (PE) is a precise tool for introducing genetic mutations in eukaryotes. Extending the efficient editing scope and mitigating undesired byproducts are possible. We introduce reverse PE (rPE), a SpCas9-directed variant that enabled DNA… read more here.
Keywords: reverse editing; rpe; window; fidelity ... See more keywords