Prime editing in plants and mammalian cells: Mechanism, achievements, limitations, and future prospects
Sign Up to like & getrecommendations! Published in 2022 at "BioEssays"
DOI: 10.1002/bies.202200032
Abstract: Clustered, regularly interspaced, short palindromic repeat (CRISPR)/CRISPR‐associated protein (CRISPR/Cas) system has revolutionized genetic research in the life sciences. Four classes of CRISPR/Cas‐derived genome editing agents, such as nuclease, base editor, recombinase, and prime editor have… read more here.
Keywords: genome editing; crispr; prime editing; plants mammalian ... See more keywords
Adenine base editing and prime editing of chemically derived hepatic progenitors rescue genetic liver disease.
Sign Up to like & getrecommendations! Published in 2021 at "Cell stem cell"
DOI: 10.1016/j.stem.2021.04.010
Abstract: DNA base editors and prime editing technology enable therapeutic in situ correction of disease-causing alleles. These techniques could have broad applications for ex vivo editing of cells prior to transplantation in a range of diseases, but… read more here.
Keywords: derived hepatic; hepatic progenitors; disease; base ... See more keywords
Prime Editing Guide RNA Design Automation Using PINE-CONE
Sign Up to like & getrecommendations! Published in 2021 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.0c00445
Abstract: CRISPR-based technologies are paramount in genome engineering and synthetic biology. Prime editing (PE) is a technology capable of installing genomic edits without double-stranded DNA breaks (DSBs) or donor DNA. Prime editing guide RNAs (pegRNAs) simultaneously… read more here.
Keywords: pine cone; guide; biology; prime editing ... See more keywords
WT-PE: Prime editing with nuclease wild-type Cas9 enables versatile large-scale genome editing
Sign Up to like & getrecommendations! Published in 2022 at "Signal Transduction and Targeted Therapy"
DOI: 10.1038/s41392-022-00936-w
Abstract: Large scale genomic aberrations including duplication, deletion, translocation, and other structural changes are the cause of a subtype of hereditary genetic disorders and contribute to onset or progress of cancer. The current prime editor, PE2,… read more here.
Keywords: large scale; prime editing; type cas9; nuclease wild ... See more keywords
Efficient generation of mouse models with the prime editing system
Sign Up to like & getrecommendations! Published in 2020 at "Cell Discovery"
DOI: 10.1038/s41421-020-0165-z
Abstract: Dear Editor, Most genetic diseases in humans are caused by singlenucleotide mutations. Although genome editing with either the CRISPR-based cytosine base editor (CBE) or the adenine base editor (ABE) holds great promise for gene correction… read more here.
Keywords: system; base; prime editing; efficiency ... See more keywords
PrimeDesign software for rapid and simplified design of prime editing guide RNAs.
Sign Up to like & getrecommendations! Published in 2021 at "Nature communications"
DOI: 10.1038/s41467-021-21337-7
Abstract: Prime editing (PE) is a versatile genome editing technology, but design of the required guide RNAs is more complex than for standard CRISPR-based nucleases or base editors. Here we describe PrimeDesign, a user-friendly, end-to-end web… read more here.
Keywords: prime editing; guide rnas; editing guide; design ... See more keywords
Predicting the efficiency of prime editing guide RNAs in human cells
Sign Up to like & getrecommendations! Published in 2020 at "Nature Biotechnology"
DOI: 10.1038/s41587-020-0677-y
Abstract: Prime editing enables the introduction of virtually any small-sized genetic change without requiring donor DNA or double-strand breaks. However, evaluation of prime editing efficiency requires time-consuming experiments, and the factors that affect efficiency have not… read more here.
Keywords: guide rnas; editing guide; prime editing; efficiency ... See more keywords
High-efficiency prime editing with optimized, paired pegRNAs in plants.
Sign Up to like & getrecommendations! Published in 2021 at "Nature biotechnology"
DOI: 10.1038/s41587-021-00868-w
Abstract: Prime editing (PE) applications are limited by low editing efficiency. Here we show that designing prime binding sites with a melting temperature of 30 °C leads to optimal performance in rice and that using two prime… read more here.
Keywords: high efficiency; editing optimized; optimized paired; prime editing ... See more keywords
Engineered pegRNAs improve prime editing efficiency.
Sign Up to like & getrecommendations! Published in 2021 at "Nature biotechnology"
DOI: 10.1038/s41587-021-01039-7
Abstract: Prime editing enables the installation of virtually any combination of point mutations, small insertions or small deletions in the DNA of living cells. A prime editing guide RNA (pegRNA) directs the prime editor protein to… read more here.
Keywords: editing efficiency; engineered pegrnas; improve prime; pegrnas improve ... See more keywords
Improved Prime Editing allows for routine predictable gene editing in Physcomitrium patens.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of experimental botany"
DOI: 10.1093/jxb/erad189
Abstract: Efficient and precise gene editing is the gold standard of any reverse genetic study. The recently developed Prime Editing approach, a modified CRISPR-Cas9 editing method, has reached the precision goal but its editing rate can… read more here.
Keywords: allows routine; gene editing; prime editing; gene ... See more keywords
Comprehensive analysis of prime editing outcomes in human embryonic stem cells
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkab1295
Abstract: Abstract Prime editing is a versatile and precise genome editing technique that can directly copy desired genetic modifications into target DNA sites without the need for donor DNA. This technique holds great promise for the… read more here.
Keywords: analysis prime; stem cells; prime editing; comprehensive analysis ... See more keywords