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   Articles with "primer" as a keyword



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Improvement and evaluation of loop-mediated isothermal amplification combined with a chromatographic flow dipstick assay and utilization in detection of Vibrio cholerae

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recommendations! Published in 2018 at "Analytical and Bioanalytical Chemistry"

DOI: 10.1007/s00216-018-1472-1

Abstract: AbstractLoop-mediated isothermal amplification (LAMP) is a specific, sensitive, and easy-to-perform nucleic acid analytical technique with wide application for diagnosis of disease. Recently, LAMP combined with use of a lateral chromatographic flow dipstick (LFD) has been… read more here.

Keywords: mediated isothermal; primer; lfd assays; isothermal amplification ... See more keywords
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Improvement of Anticorrosion Properties of Epoxy Primer Coating on Aluminum Alloy 2024-T3 by Thiosemicarbazone Derivatives

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recommendations! Published in 2019 at "JOM"

DOI: 10.1007/s11837-019-03615-4

Abstract: The effect of adding thiosemicarbazone derivatives on the anticorrosion properties of epoxy primer coatings on aluminum alloy 2024-T3 was investigated. (E)-2-(2,4-dihydroxybenzylidene)hydrazinecarbothioamide (2,4-DHC), (E)-2-(3,4-dihydroxybenzylidene)hydrazinecarbothioamide (3,4-DHC), and (E)-2-(2,3,4-dihydroxybenzylidene)hydrazinecarbothioamide (2,3,4-THC) were supplemented to epoxy primer that is generally… read more here.

Keywords: thiosemicarbazone derivatives; primer coating; epoxy primer; anticorrosion properties ... See more keywords
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Primer development of toll-like receptor genes in the vulnerable Chinese egret (Egretta eulophotes) and its applicability in Ardeidae

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recommendations! Published in 2020 at "Conservation Genetics Resources"

DOI: 10.1007/s12686-020-01179-z

Abstract: Toll-like receptor (TLR) genes have recently been used to assess the adaptive genetic diversity under natural selection. Here, we newly developed a set of primer pairs for amplifying TLR genes (TLR1LB, TLR2A, TLR3, TLR4, TLR5,… read more here.

Keywords: toll like; egret egretta; primer; vulnerable chinese ... See more keywords
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Highly specific real-time quantification of diverse microRNAs in human samples using universal primer set frame.

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recommendations! Published in 2018 at "Analytical biochemistry"

DOI: 10.1016/j.ab.2017.12.004

Abstract: In this study, one group of universal primer set frame, composed by one reverse transcription (RT) primer frame and a pair of quantitative real-time polymerase chain reaction (qRT-PCR) primer frames, was elaborately screened and designed… read more here.

Keywords: primer; real time; universal primer; frame ... See more keywords
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One-step genotyping method in CRISPR based on short inner primer-assisted, tetra primer-paired amplifications.

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recommendations! Published in 2020 at "Molecular and cellular probes"

DOI: 10.1016/j.mcp.2020.101675

Abstract: Base editors and prime editors induce precise DNA modifications over one or several nucleotides in eukaryotic cells. The T7E1 assay has been widely adopted for the assessment of genome editing, but it has several limitations… read more here.

Keywords: inner; primer; primer assisted; assisted tetra ... See more keywords
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Single-Step and Highly Sensitive Imaging of Exosomal PD-L1 through Aptamer-Activated Cascade Primer Exchange Reaction-Generated Branched DNA Nanostructures.

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recommendations! Published in 2022 at "ACS sensors"

DOI: 10.1021/acssensors.2c01614

Abstract: Exosomal PD-L1 plays a critical role in tumor progress and immunotherapy. However, accurately analyzing exosomal PD-L1 is greatly limited by the small-sized and free-floating nature of exosomes and the few proteins each exosome carries. We… read more here.

Keywords: branched dna; single step; generated branched; imaging exosomal ... See more keywords
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H-Bond Templated Oligomer Synthesis Using a Covalent Primer

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recommendations! Published in 2022 at "Journal of the American Chemical Society"

DOI: 10.1021/jacs.2c08119

Abstract: Template-directed synthesis of nucleic acids in the polymerase chain reaction is based on the use of a primer, which is elongated in the replication process. The attachment of a high affinity primer to the end… read more here.

Keywords: template; synthesis; primer; phosphine oxide ... See more keywords
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Structural Rationale for the Enhanced Catalysis of Nonenzymatic RNA Primer Extension by a Downstream Oligonucleotide

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recommendations! Published in 2018 at "Journal of the American Chemical Society"

DOI: 10.1021/jacs.7b11750

Abstract: Nonenzymatic RNA primer extension by activated mononucleotides has long served as a model for the study of prebiotic RNA copying. We have recently shown that the rate of primer extension is greatly enhanced by the… read more here.

Keywords: primer; rna; downstream oligonucleotide; downstream ... See more keywords
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The combined DNA and RNA synthetic capabilities of archaeal DNA primase facilitate primer hand-off to the replicative DNA polymerase

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recommendations! Published in 2022 at "Nature Communications"

DOI: 10.1038/s41467-022-28093-2

Abstract: Replicative DNA polymerases cannot initiate DNA synthesis de novo and rely on dedicated RNA polymerases, primases, to generate a short primer. This primer is then extended by the DNA polymerase. In diverse archaeal species, the… read more here.

Keywords: dna; dna polymerase; primer; primase ... See more keywords
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Primerdiffer: a python command-line module for large-scale primer design in haplotype genotyping

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recommendations! Published in 2023 at "Bioinformatics"

DOI: 10.1093/bioinformatics/btad188

Abstract: Abstract Motivation Primer design is a routine practice for modern molecular biology labs. Bioinformatics tools like primer3 and primer-blast have standardized the primer design for a specific region. However, large-scale primer design, especially for genome-wide… read more here.

Keywords: primer design; command line; large scale; primer ... See more keywords
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sRNAPrimerDB: comprehensive primer design and search web service for small non-coding RNAs

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recommendations! Published in 2019 at "Bioinformatics"

DOI: 10.1093/bioinformatics/bty852

Abstract: MOTIVATION Small non-coding RNAs (ncRNAs), especially microRNAs (miRNAs) and piwi-interacting RNAs (piRNAs), play key roles in many biological processes. However, only a few tools can be used to develop the optimal primer or probe design… read more here.

Keywords: primer; coding rnas; small non; non coding ... See more keywords