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   Articles with "primer" as a keyword



Reversible Demountable Coating Films Composed of Trifunctional Liquid Anthracene Compounds and Evaluation of Their Adhesive Properties

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recommendations! Published in 2024 at "Macromolecular Chemistry and Physics"

DOI: 10.1002/macp.202400217

Abstract: Technologies that facilitate recycling are attracting considerable interest from the perspective of resource constraints. In the present study, degradable primer films are to facilitate the removal of strongly bonded joints. This film consists of a… read more here.

Keywords: liquid; primer; reversible demountable; primer film ... See more keywords

Improvement and evaluation of loop-mediated isothermal amplification combined with a chromatographic flow dipstick assay and utilization in detection of Vibrio cholerae

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recommendations! Published in 2018 at "Analytical and Bioanalytical Chemistry"

DOI: 10.1007/s00216-018-1472-1

Abstract: AbstractLoop-mediated isothermal amplification (LAMP) is a specific, sensitive, and easy-to-perform nucleic acid analytical technique with wide application for diagnosis of disease. Recently, LAMP combined with use of a lateral chromatographic flow dipstick (LFD) has been… read more here.

Keywords: mediated isothermal; primer; lfd assays; isothermal amplification ... See more keywords

Improvement of Anticorrosion Properties of Epoxy Primer Coating on Aluminum Alloy 2024-T3 by Thiosemicarbazone Derivatives

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recommendations! Published in 2019 at "JOM"

DOI: 10.1007/s11837-019-03615-4

Abstract: The effect of adding thiosemicarbazone derivatives on the anticorrosion properties of epoxy primer coatings on aluminum alloy 2024-T3 was investigated. (E)-2-(2,4-dihydroxybenzylidene)hydrazinecarbothioamide (2,4-DHC), (E)-2-(3,4-dihydroxybenzylidene)hydrazinecarbothioamide (3,4-DHC), and (E)-2-(2,3,4-dihydroxybenzylidene)hydrazinecarbothioamide (2,3,4-THC) were supplemented to epoxy primer that is generally… read more here.

Keywords: thiosemicarbazone derivatives; primer coating; epoxy primer; anticorrosion properties ... See more keywords

Primer development of toll-like receptor genes in the vulnerable Chinese egret (Egretta eulophotes) and its applicability in Ardeidae

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recommendations! Published in 2020 at "Conservation Genetics Resources"

DOI: 10.1007/s12686-020-01179-z

Abstract: Toll-like receptor (TLR) genes have recently been used to assess the adaptive genetic diversity under natural selection. Here, we newly developed a set of primer pairs for amplifying TLR genes (TLR1LB, TLR2A, TLR3, TLR4, TLR5,… read more here.

Keywords: toll like; egret egretta; primer; vulnerable chinese ... See more keywords

Highly specific real-time quantification of diverse microRNAs in human samples using universal primer set frame.

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recommendations! Published in 2018 at "Analytical biochemistry"

DOI: 10.1016/j.ab.2017.12.004

Abstract: In this study, one group of universal primer set frame, composed by one reverse transcription (RT) primer frame and a pair of quantitative real-time polymerase chain reaction (qRT-PCR) primer frames, was elaborately screened and designed… read more here.

Keywords: primer; real time; universal primer; frame ... See more keywords

One-step genotyping method in CRISPR based on short inner primer-assisted, tetra primer-paired amplifications.

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recommendations! Published in 2020 at "Molecular and cellular probes"

DOI: 10.1016/j.mcp.2020.101675

Abstract: Base editors and prime editors induce precise DNA modifications over one or several nucleotides in eukaryotic cells. The T7E1 assay has been widely adopted for the assessment of genome editing, but it has several limitations… read more here.

Keywords: inner; primer; primer assisted; assisted tetra ... See more keywords

Single-Step and Highly Sensitive Imaging of Exosomal PD-L1 through Aptamer-Activated Cascade Primer Exchange Reaction-Generated Branched DNA Nanostructures.

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recommendations! Published in 2022 at "ACS sensors"

DOI: 10.1021/acssensors.2c01614

Abstract: Exosomal PD-L1 plays a critical role in tumor progress and immunotherapy. However, accurately analyzing exosomal PD-L1 is greatly limited by the small-sized and free-floating nature of exosomes and the few proteins each exosome carries. We… read more here.

Keywords: branched dna; single step; generated branched; imaging exosomal ... See more keywords

H-Bond Templated Oligomer Synthesis Using a Covalent Primer

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recommendations! Published in 2022 at "Journal of the American Chemical Society"

DOI: 10.1021/jacs.2c08119

Abstract: Template-directed synthesis of nucleic acids in the polymerase chain reaction is based on the use of a primer, which is elongated in the replication process. The attachment of a high affinity primer to the end… read more here.

Keywords: template; synthesis; primer; phosphine oxide ... See more keywords

Structural Rationale for the Enhanced Catalysis of Nonenzymatic RNA Primer Extension by a Downstream Oligonucleotide

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recommendations! Published in 2018 at "Journal of the American Chemical Society"

DOI: 10.1021/jacs.7b11750

Abstract: Nonenzymatic RNA primer extension by activated mononucleotides has long served as a model for the study of prebiotic RNA copying. We have recently shown that the rate of primer extension is greatly enhanced by the… read more here.

Keywords: primer; rna; downstream oligonucleotide; downstream ... See more keywords
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The combined DNA and RNA synthetic capabilities of archaeal DNA primase facilitate primer hand-off to the replicative DNA polymerase

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recommendations! Published in 2022 at "Nature Communications"

DOI: 10.1038/s41467-022-28093-2

Abstract: Replicative DNA polymerases cannot initiate DNA synthesis de novo and rely on dedicated RNA polymerases, primases, to generate a short primer. This primer is then extended by the DNA polymerase. In diverse archaeal species, the… read more here.

Keywords: dna; dna polymerase; primer; primase ... See more keywords

Olivar: towards automated variant aware primer design for multiplex tiled amplicon sequencing of pathogens

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recommendations! Published in 2024 at "Nature Communications"

DOI: 10.1038/s41467-024-49957-9

Abstract: Tiled amplicon sequencing has served as an essential tool for tracking the spread and evolution of pathogens. Over 15 million complete SARS-CoV-2 genomes are now publicly available, most sequenced and assembled via tiled amplicon sequencing.… read more here.

Keywords: primer; automated variant; amplicon sequencing; variant aware ... See more keywords