One-step genotyping method in CRISPR based on short inner primer-assisted, tetra primer-paired amplifications.
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DOI: 10.1016/j.mcp.2020.101675
Abstract: Base editors and prime editors induce precise DNA modifications over one or several nucleotides in eukaryotic cells. The T7E1 assay has been widely adopted for the assessment of genome editing, but it has several limitations… read more here.
Keywords: inner; primer; primer assisted; assisted tetra ... See more keywords