Multiobjective optimization-driven primer design mechanism: towards user-specified parameters of PCR primer
Sign Up to like & getrecommendations! Published in 2022 at "Briefings in bioinformatics"
DOI: 10.1093/bib/bbac121
Abstract: Primers are critical for polymerase chain reaction (PCR) and influence PCR experimental outcomes. Designing numerous combinations of forward and reverse primers involves various primer constraints, posing a computational challenge. Most PCR primer design methods limit… read more here.
Keywords: fitness functions; specified parameters; user specified; primer design ... See more keywords
Primerdiffer: a python command-line module for large-scale primer design in haplotype genotyping
Sign Up to like & getrecommendations! Published in 2023 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btad188
Abstract: Abstract Motivation Primer design is a routine practice for modern molecular biology labs. Bioinformatics tools like primer3 and primer-blast have standardized the primer design for a specific region. However, large-scale primer design, especially for genome-wide… read more here.
Keywords: primer design; command line; large scale; primer ... See more keywords
Primerize-2D: automated primer design for RNA multidimensional chemical mapping.
Sign Up to like & getrecommendations! Published in 2017 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btw814
Abstract: Summary Rapid RNA synthesis of comprehensive single mutant libraries and targeted multiple mutant libraries is enabling new multidimensional chemical approaches to solve RNA structures. PCR assembly of DNA templates and in vitro transcription allow synthesis… read more here.
Keywords: multidimensional chemical; primer design; automated primer; primerize automated ... See more keywords
Primer design for quantitative real-time PCR for the emerging Coronavirus SARS-CoV-2
Sign Up to like & getrecommendations! Published in 2020 at "Theranostics"
DOI: 10.7150/thno.47649
Abstract: In December 2019, a new coronavirus disease (COVID-19) outbreak occurred in Wuhan, China. Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), which is the seventh coronavirus known to infect humans, is highly contagious and has rapidly expanded worldwide… read more here.
Keywords: coronavirus sars; primer design; coronavirus; new coronavirus ... See more keywords