Structural Rationale for the Enhanced Catalysis of Nonenzymatic RNA Primer Extension by a Downstream Oligonucleotide
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DOI: 10.1021/jacs.7b11750
Abstract: Nonenzymatic RNA primer extension by activated mononucleotides has long served as a model for the study of prebiotic RNA copying. We have recently shown that the rate of primer extension is greatly enhanced by the… read more here.
Keywords: primer; rna; downstream oligonucleotide; downstream ... See more keywords
A Model for the Emergence of RNA from a Prebiotically Plausible Mixture of Ribonucleotides, Arabinonucleotides, and 2′-Deoxynucleotides
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DOI: 10.1021/jacs.9b11239
Abstract: The abiotic synthesis of ribonucleotides is thought to have been an essential step toward the emergence of the RNA world. However, it is likely that the prebiotic synthesis of ribonucleotides was accompanied by the simultaneous… read more here.
Keywords: rna; synthesis; emergence rna; primer extension ... See more keywords
Optimizing genome editing strategy by primer-extension-mediated sequencing
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DOI: 10.1038/s41421-019-0088-8
Abstract: Efficient and precise genome editing is essential for clinical applications and generating animal models, which requires engineered nucleases with high editing ability while low off-target activity. Here we present a high-throughput sequencing method, primer-extension-mediated sequencing… read more here.
Keywords: genome editing; mediated sequencing; extension mediated; target ... See more keywords
Auto-cycling primer extension for amplified microRNA detection.
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DOI: 10.1039/d2cc01784a
Abstract: The current polymerase-based nucleic acid amplification techniques cannot maintain continuous polymerization reactions unless by changing the temperature or adding additional reagents (e.g. the second enzyme or betaine), which undoubtedly increases the cost and operation steps.… read more here.
Keywords: cycling primer; primer extension; detection; auto cycling ... See more keywords
Competition between bridged dinucleotides and activated mononucleotides determines the error frequency of nonenzymatic RNA primer extension
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DOI: 10.1093/nar/gkab173
Abstract: Abstract Nonenzymatic copying of RNA templates with activated nucleotides is a useful model for studying the emergence of heredity at the origin of life. Previous experiments with defined-sequence templates have pointed to the poor fidelity… read more here.
Keywords: bridged dinucleotides; activated mononucleotides; primer; rna ... See more keywords
Visualizing primer extension without enzymes
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DOI: 10.7554/elife.37926
Abstract: X-ray crystallography has been used to observe the synthesis of RNA in the absence of enzymes with atomic resolution. read more here.
Keywords: without enzymes; visualizing primer; primer extension; extension without ... See more keywords