The Impact of Activating Agents on Non‐Enzymatic Nucleic Acid Extension Reactions
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DOI: 10.1002/cbic.202300859
Abstract: Non‐enzymatic template‐directed primer extension is increasingly being studied for the production of RNA and DNA. These reactions benefit from producing RNA or DNA in an aqueous, protecting group free system, without the need for expensive… read more here.
Keywords: non enzymatic; template directed; enzymatic template; extension ... See more keywords
Catalytic Metal Ion–Substrate Coordination during Nonenzymatic RNA Primer Extension
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DOI: 10.1021/jacs.4c00323
Abstract: Nonenzymatic template-directed RNA copying requires catalysis by divalent metal ions. The primer extension reaction involves the attack of the primer 3′-hydroxyl on the adjacent phosphate of a 5′-5′-imidazolium-bridged dinucleotide substrate. However, the nature of the… read more here.
Keywords: primer extension; catalytic metal; metal; ion ... See more keywords
Structural Rationale for the Enhanced Catalysis of Nonenzymatic RNA Primer Extension by a Downstream Oligonucleotide
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DOI: 10.1021/jacs.7b11750
Abstract: Nonenzymatic RNA primer extension by activated mononucleotides has long served as a model for the study of prebiotic RNA copying. We have recently shown that the rate of primer extension is greatly enhanced by the… read more here.
Keywords: primer; rna; downstream oligonucleotide; downstream ... See more keywords
A Model for the Emergence of RNA from a Prebiotically Plausible Mixture of Ribonucleotides, Arabinonucleotides, and 2′-Deoxynucleotides
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DOI: 10.1021/jacs.9b11239
Abstract: The abiotic synthesis of ribonucleotides is thought to have been an essential step toward the emergence of the RNA world. However, it is likely that the prebiotic synthesis of ribonucleotides was accompanied by the simultaneous… read more here.
Keywords: rna; synthesis; emergence rna; primer extension ... See more keywords
Optimizing genome editing strategy by primer-extension-mediated sequencing
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DOI: 10.1038/s41421-019-0088-8
Abstract: Efficient and precise genome editing is essential for clinical applications and generating animal models, which requires engineered nucleases with high editing ability while low off-target activity. Here we present a high-throughput sequencing method, primer-extension-mediated sequencing… read more here.
Keywords: genome editing; mediated sequencing; extension mediated; target ... See more keywords
Auto-cycling primer extension for amplified microRNA detection.
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DOI: 10.1039/d2cc01784a
Abstract: The current polymerase-based nucleic acid amplification techniques cannot maintain continuous polymerization reactions unless by changing the temperature or adding additional reagents (e.g. the second enzyme or betaine), which undoubtedly increases the cost and operation steps.… read more here.
Keywords: cycling primer; primer extension; detection; auto cycling ... See more keywords
Competition between bridged dinucleotides and activated mononucleotides determines the error frequency of nonenzymatic RNA primer extension
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DOI: 10.1093/nar/gkab173
Abstract: Abstract Nonenzymatic copying of RNA templates with activated nucleotides is a useful model for studying the emergence of heredity at the origin of life. Previous experiments with defined-sequence templates have pointed to the poor fidelity… read more here.
Keywords: bridged dinucleotides; activated mononucleotides; primer; rna ... See more keywords
Constraints on the emergence of RNA through non-templated primer extension with mixtures of potentially prebiotic nucleotides
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DOI: 10.1101/2024.01.21.576316
Abstract: The emergence of RNA on the early Earth is likely to have been influenced by a series of chemical and physical processes that acted to filter out various alternative nucleic acids. For example, UV photostability… read more here.
Keywords: threo nucleotides; emergence rna; non templated; extension ... See more keywords
Visualizing primer extension without enzymes
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DOI: 10.7554/elife.37926
Abstract: X-ray crystallography has been used to observe the synthesis of RNA in the absence of enzymes with atomic resolution. read more here.
Keywords: without enzymes; visualizing primer; primer extension; extension without ... See more keywords