Two new superior primer pairs for universal detection of Xylella spp. in conventional PCR and TaqMan quantitative real-time PCR.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2021.106321
Abstract: X. fastidiosa causes many economically important plant diseases such as Pierce's disease of grapevine, citrus variegated chlorosis disease, and olive quick decline syndrome. Another species in the same genus, Xylella taiwanensis, causes pear leaf scorch.… read more here.
Keywords: spp conventional; taqman; pcr; primer pairs ... See more keywords
Identification of S genotypes in loquat (Eriobotrya japonica Lindl.) based on allele specific PCR
Sign Up to like & getrecommendations! Published in 2017 at "Scientia Horticulturae"
DOI: 10.1016/j.scienta.2017.07.050
Abstract: Abstract Loquat (Eriobotrya japonica Lindl) is an evergreen fruit tree and belongs to Maloideae in the Rosaceae, which carries the RNase-dependent gametophytic self-incompatibility system (GSI). Use of appropriate pollinator trees is essential for increasing fruit… read more here.
Keywords: specific pcr; loquat eriobotrya; eriobotrya japonica; allele specific ... See more keywords
Identifying group‐specific primers for environmental Heterolobosa by high‐throughput sequencing
Sign Up to like & getrecommendations! Published in 2022 at "Microbial Biotechnology"
DOI: 10.1111/1751-7915.14080
Abstract: Diversity of Heterolobosea (Excavata) in environments is poorly understood despite their ecological occurrence and health‐associated risk, partly because this group tends to be under‐covered by most universal eukaryotic primers used for sequencing. To overcome the… read more here.
Keywords: group; high throughput; throughput sequencing; primer pairs ... See more keywords
In-Silico Detection of Oral Prokaryotic Species With Highly Similar 16S rRNA Sequence Segments Using Different Primer Pairs
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Cellular and Infection Microbiology"
DOI: 10.3389/fcimb.2021.770668
Abstract: Although clustering by operational taxonomic units (OTUs) is widely used in the oral microbial literature, no research has specifically evaluated the extent of the limitations of this sequence clustering-based method in the oral microbiome. Consequently,… read more here.
Keywords: sequence segments; sequence; primer; oral species ... See more keywords
Fine-scale evaluation of two standard 16S rRNA gene amplicon primer pairs for analysis of total prokaryotes and archaeal nitrifiers in differently managed soils
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2023.1140487
Abstract: The advance of high-throughput molecular biology tools allows in-depth profiling of microbial communities in soils, which possess a high diversity of prokaryotic microorganisms. Amplicon-based sequencing of 16S rRNA genes is the most common approach to… read more here.
Keywords: evaluation; 16s rrna; primer; primer pairs ... See more keywords
Development and Characterization of EST-SSR Markers From RNA-Seq Data in Phyllostachys violascens
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2019.00050
Abstract: Bamboo are woody grass species containing important economic and ecological values. Lei bamboo (Phyllostachys violascens) is a kind of shoot-producing bamboo species with the highest economic yield per unit area. However, identifying different varieties of… read more here.
Keywords: primer pairs; est ssr; phyllostachys violascens; lei bamboo ... See more keywords