Highly specific real-time quantification of diverse microRNAs in human samples using universal primer set frame.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2017.12.004
Abstract: In this study, one group of universal primer set frame, composed by one reverse transcription (RT) primer frame and a pair of quantitative real-time polymerase chain reaction (qRT-PCR) primer frames, was elaborately screened and designed… read more here.
Keywords: primer; real time; universal primer; frame ... See more keywords
Discrimination of raw material species in mixed seafood products (surimi) using the next generation sequencing method
Sign Up to like & getrecommendations! Published in 2020 at "Food bioscience"
DOI: 10.1016/j.fbio.2020.100786
Abstract: Abstract The increased international trade and worldwide consumption of seafood has increased the possibility of economic fraud. Particularly, the increased development of processed seafood has restricted the application of classical identification methods. DNA matabarcoding is… read more here.
Keywords: raw material; seafood products; seafood; discrimination raw ... See more keywords
Reverse transcription-PCR using a primer set targeting the 3D region detects foot-and-mouth disease virus with high sensitivity.
Sign Up to like & getrecommendations! Published in 2019 at "Transboundary and emerging diseases"
DOI: 10.1111/tbed.13202
Abstract: The potential of foot-and-mouth disease (FMD) to spread extensively means that rapid and accurate methods are needed for its diagnosis. Therefore, reverse transcription-PCR (RT-PCR) plays an important diagnostic role. Here, we designed the primer set… read more here.
Keywords: foot mouth; pcr; primer set; pcr assays ... See more keywords
Development and validation of a real-time PCR assay for the detection of clinical acanthamoebae
Sign Up to like & getrecommendations! Published in 2017 at "BMC Research Notes"
DOI: 10.1186/s13104-017-2666-x
Abstract: BackgroundSuboptimal agreement between molecular assays for the detection of Acanthamoeba spp. in clinical specimens has been demonstrated, and poor assay sensitivity directly imperils the vision of those affected by amoebic keratitis (AK) through delayed diagnosis.… read more here.
Keywords: detection; real time; time pcr; pcr assay ... See more keywords
Comparing eDNA metabarcoding primers for assessing fish communities in a biodiverse estuary
Sign Up to like & getrecommendations! Published in 2022 at "PLoS ONE"
DOI: 10.1371/journal.pone.0266720
Abstract: Metabarcoding of environmental DNA is increasingly used for biodiversity assessments in aquatic communities. The efficiency and outcome of these efforts are dependent upon either de novo primer design or selecting an appropriate primer set from… read more here.
Keywords: 12s primer; riaz 12s; primer; metabarcoding primers ... See more keywords
Human Alphacoronavirus Universal Primers for Genome Amplification and Sequencing
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.789665
Abstract: Rapid and accurate sequencing covering the entire genome is essential to identify genetic variations of viral pathogens. However, due to the low viral titers in clinical samples, certain amplification steps are required for viral genome… read more here.
Keywords: entire genome; genome amplification; universal primers; primer set ... See more keywords
Pseudomonas aeruginosa Detection Using Conventional PCR and Quantitative Real-Time PCR Based on Species-Specific Novel Gene Targets Identified by Pangenome Analysis
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.820431
Abstract: Mining novel specific molecular targets and establishing efficient identification methods are significant for detecting Pseudomonas aeruginosa, which can enable P. aeruginosa tracing in food and water. Pangenome analysis was used to analyze the whole genomic… read more here.
Keywords: ucbpp pa14; detection; species specific; primer set ... See more keywords
New Insights Into Nematode DNA-metabarcoding as Revealed by the Characterization of Artificial and Spiked Nematode Communities
Sign Up to like & getrecommendations! Published in 2019 at "Diversity"
DOI: 10.3390/d11040052
Abstract: Nematodes are ideal biological indicators to monitor soil biodiversity and ecosystem functioning. For this reason, they have been receiving increasing attention from a broad range of scientists. The main method to characterize soil nematode communities… read more here.
Keywords: nematode communities; new insights; nematode species; primer set ... See more keywords
Genomic analysis of red-tide water bloomed with Heterosigma akashiwo in Geoje
Sign Up to like & getrecommendations! Published in 2018 at "PeerJ"
DOI: 10.7717/peerj.4854
Abstract: Microbial community structures of harmful algal bloom (HAB) caused by Heterosigma akashiwo in Geoje were analyzed using the MiSeq platform. To analyze phytoplankton communities without cross-reactivity with predominant bacteria, a new phytoplankton-specific 23S universal primer… read more here.
Keywords: primer; heterosigma akashiwo; universal primer; bloom ... See more keywords