One‐locus‐several‐primers: A strategy to improve the taxonomic and haplotypic coverage in diet metabarcoding studies
Sign Up to like & getrecommendations! Published in 2019 at "Ecology and Evolution"
DOI: 10.1002/ece3.5063
Abstract: Abstract In diet metabarcoding analyses, insufficient taxonomic coverage of PCR primer sets generates false negatives that may dramatically distort biodiversity estimates. In this paper, we investigated the taxonomic coverage and complementarity of three cytochrome c… read more here.
Keywords: diet metabarcoding; locus several; strategy; coverage ... See more keywords
In silico and empirical evaluation of twelve metabarcoding primer sets for insectivorous diet analyses
Sign Up to like & getrecommendations! Published in 2020 at "Ecology and Evolution"
DOI: 10.1002/ece3.6362
Abstract: Abstract During the most recent decade, environmental DNA metabarcoding approaches have been both developed and improved to minimize the biological and technical biases in these protocols. However, challenges remain, notably those relating to primer design.… read more here.
Keywords: silico empirical; empirical evaluation; bat; evaluation twelve ... See more keywords
Development of molecular markers to distinguish between morphologically similar edible plants and poisonous plants using real-time PCR assay.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of the science of food and agriculture"
DOI: 10.1002/jsfa.10711
Abstract: BACKGROUND Due to a similar appearance between edible and poisonous plants, a total of 42 patients ingesting poisonous plants have occurred from 2013 to 2017 in Korea. We developed species-specific primer sets of three of… read more here.
Keywords: real time; time pcr; primer sets; plants using ... See more keywords
High throughput quantification of the functional genes associated with RDX biodegradation using the SmartChip real-time PCR system
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DOI: 10.1007/s00253-019-10022-x
Abstract: The explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) is a contaminant at many military sites. RDX bioremediation as a clean-up approach has been gaining popularity because of cost benefits compared to other methods. RDX biodegradation has primarily been linked… read more here.
Keywords: functional genes; biodegradation; pcr; rdx biodegradation ... See more keywords
Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR
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DOI: 10.1007/s00253-022-11822-4
Abstract: Abstract The pandemic of coronavirus disease 2019 (COVID-19) continues to threaten public health. For developing countries where vaccines are still in shortage, cheaper alternative molecular methods for SARS-CoV-2 identification can be crucial to prevent the… read more here.
Keywords: sybr green; primer sets; sars cov; based qpcr ... See more keywords
Primer set 2.0 for highly parallel qPCR array targeting antibiotic resistance genes and mobile genetic elements
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DOI: 10.1093/femsec/fiy130
Abstract: ABSTRACT The high-throughput antibiotic resistance gene (ARG) qPCR array, initially published in 2012, is increasingly used to quantify resistance and mobile determinants in environmental matrices. Continued utility of the array; however, necessitates improvements such as… read more here.
Keywords: antibiotic resistance; primer sets; primer; qpcr array ... See more keywords
Comparing eDNA metabarcoding primers for assessing fish communities in a biodiverse estuary
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DOI: 10.1371/journal.pone.0266720
Abstract: Metabarcoding of environmental DNA is increasingly used for biodiversity assessments in aquatic communities. The efficiency and outcome of these efforts are dependent upon either de novo primer design or selecting an appropriate primer set from… read more here.
Keywords: 12s primer; riaz 12s; primer; metabarcoding primers ... See more keywords
Comparison five primer sets from different genome region of COVID-19 for detection of virus infection by conventional RT-PCR
Sign Up to like & getrecommendations! Published in 2020 at "Iranian Journal of Microbiology"
DOI: 10.18502/ijm.v12i3.3234
Abstract: Background and Objectives: The new beta-coronavirus, which caused Severe Acute Respiratory Coronavirus-2 Syndrome (SARS-CoV-2), a major respiratory outbreak in Wuhan, China in December 2019, is now prevalent in many countries around the world. Identifying PCR-based… read more here.
Keywords: comparison five; primer sets; conventional pcr; sars cov ... See more keywords