Downstream Oligonucleotides Strongly Enhance the Affinity of GMP to RNA Primer–Template Complexes
Sign Up to like & getrecommendations! Published in 2017 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.6b09760
Abstract: Origins of life hypotheses often invoke a transitional phase of nonenzymatic template-directed RNA replication prior to the emergence of ribozyme-catalyzed copying of genetic information. Here, using NMR and ITC, we interrogate the binding affinity of… read more here.
Keywords: affinity; template; gmp; primer template ... See more keywords
A prolific and robust whole-genome genotyping method using PCR amplification via primer-template mismatched annealing.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of integrative plant biology"
DOI: 10.1111/jipb.13395
Abstract: Whole-genome genotyping methods are important for breeding. However, it has been challenging to develop a robust method for simultaneous foreground and background genotyping that can easily be adapted to different genes and species. In our… read more here.
Keywords: primer; genome; method; primer template ... See more keywords
Translocation pause of remdesivir-containing primer/template RNA duplex within SARS-CoV-2’s RNA polymerase complexes
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2022.999291
Abstract: The mechanism of remdesivir incorporation into the RNA primer by the RNA-dependent RNA polymerase (RdRp) of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) remains to be fully established at the molecular level. Here, we compare molecular… read more here.
Keywords: polymerase; template rna; rna polymerase; rna ... See more keywords
Selection of Primer–Template Sequences That Bind with Enhanced Affinity to Vaccinia Virus E9 DNA Polymerase
Sign Up to like & getrecommendations! Published in 2022 at "Viruses"
DOI: 10.3390/v14020369
Abstract: A modified SELEX (Systematic Evolution of Ligands by Exponential Enrichment) pr,otocol (referred to as PT SELEX) was used to select primer–template (P/T) sequences that bound to the vaccinia virus polymerase catalytic subunit (E9) with enhanced… read more here.
Keywords: dna; sequence; vaccinia virus; primer template ... See more keywords