Probing protein nanopores with poly(ethylene glycol)s
Sign Up to like & getrecommendations! Published in 2022 at "PROTEOMICS"
DOI: 10.1002/pmic.202100055
Abstract: Neutral water‐soluble poly(ethylene glycol)s (PEGs) have been extensively explored in protein nanopore research for the past several decades. The principal use of PEGs is to investigate the membrane protein ion channel physical characteristics and transport… read more here.
Keywords: protein nanopores; poly ethylene; ethylene glycol; protein ... See more keywords
Probing Protein Folding and Interaction in Live Cells using Osmotic Perturbations
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.319
Abstract: Cells rapidly change their volume in both optimal conditions and under duress, causing changes to their internal solute concentrations. Proteins are known to respond to such fluctuations in solution concentrations even faster, well before the… read more here.
Keywords: protein; probing protein; volume; live cells ... See more keywords
Probing Protein 4'-Phosphopantetheinylation in Single Living Cells.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.3c00080
Abstract: 4'-Phosphopantetheinylation (4PPTylation) of proteins, which is derived from the hydrolysis of coenzyme A (CoA), is an essential post-translational modification participating in biosynthetic and metabolic pathways. However, due to the lack of specific recognition ligands as… read more here.
Keywords: phosphopantetheinylation; aldh1l1 single; single living; living cells ... See more keywords
Probing protein interaction networks by combining MS-based proteomics and structural data integration.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.0c00066
Abstract: Protein-protein interactions play a major role in the molecular machinery of life and various techniques such as AP-MS are dedicated to their identification. However, those techniques return lists of proteins devoid of organizational structure, not… read more here.
Keywords: protein interaction; interaction networks; probing protein; combining based ... See more keywords
Probing protein ubiquitination in live cells
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac805
Abstract: The reversible attachment of ubiquitin governs the interaction, activity and degradation of proteins whereby the type and target of this conjugation determine the biological response. The investigation of this complex and multi-faceted protein ubiquitination mostly… read more here.
Keywords: ubiquitination live; live cells; protein ubiquitination; ubiquitination ... See more keywords