A Processive Protein Chimera Introduces Mutations across Defined DNA Regions In Vivo.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.8b04001
Abstract: Laboratory time scale evolution in vivo relies on the generation of large, mutationally diverse gene libraries to rapidly explore biomolecule sequence landscapes. Traditional global mutagenesis methods are problematic because they introduce many off-target mutations that… read more here.
Keywords: dna regions; dna; defined dna; protein chimera ... See more keywords
Single turnover transient state kinetics reveals processive protein unfolding catalyzed by Escherichia coli ClpB
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DOI: 10.7554/elife.99052
Abstract: E. coli ClpB, and S. cerevisiae Hsp104 are AAA+ motor proteins essential for proteome maintenance and thermal tolerance. ClpB and Hsp104 have been proposed to extract a polypeptide from an aggregate and processively translocate the… read more here.
Keywords: clpb; protein unfolding; processive protein; single turnover ... See more keywords