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   Articles with "product specificity" as a keyword



Mutational analyses for product specificity of YjiC towards α-mangostin mono-glucoside.

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recommendations! Published in 2018 at "Enzyme and microbial technology"

DOI: 10.1016/j.enzmictec.2018.08.001

Abstract: Glycosyltransferases (GTs) are key enzymes for the post-modification of secondary metabolites in drug development processes. In our prior research, an one-pot enzymatic system produced α-mangostin 3,6-di-O-β-D-glucopyranoside (Mg1) at a higher proportion using wild-type glycosyltransferase (YjiC)… read more here.

Keywords: product specificity; product; h298s h298c; wild type ... See more keywords

Mutations in Amino Acid Residues of Limosilactobacillus reuteri 121 GtfB 4,6-α-Glucanotransferase that Affect Reaction and Product Specificity.

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recommendations! Published in 2022 at "Journal of agricultural and food chemistry"

DOI: 10.1021/acs.jafc.1c07618

Abstract: Limosilactobacillus reuteri 121 4,6-α-glucanotransferase (Lr121 4,6-α-GTase), belonging to the glycosyl hydrolase (GH) 70 GtfB subfamily, converts starch and maltodextrins into linear isomalto/malto polysaccharides (IMMPs) with consecutive (α1 → 6) linkages. The recent elucidation of its… read more here.

Keywords: product specificity; reaction product; limosilactobacillus reuteri; product ... See more keywords

Discovery and Characterization of a Novel Chitosanase from Paenibacillus dendritiformis by Phylogeny-Based Enzymatic Product Specificity Prediction.

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recommendations! Published in 2018 at "Journal of agricultural and food chemistry"

DOI: 10.1021/acs.jafc.7b06067

Abstract: In the process of genome mining for novel chitosanases by phylogeny-based enzymatic product specificity prediction, a gene named Csn-PD from Paenibacillus dendritiformis was discovered. The enzyme was classified as a member of the GH46 family… read more here.

Keywords: phylogeny based; product specificity; product; enzymatic product ... See more keywords

Structure-Based Engineering of a Maltooligosaccharide-forming Amylase to Enhance Product Specificity.

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recommendations! Published in 2020 at "Journal of agricultural and food chemistry"

DOI: 10.1021/acs.jafc.9b07234

Abstract: Maltooligosaccharide-forming amylases (MFAses) are promising tools for a variety of food industry applications because they convert starch into functional maltooligosaccharides. The MFAse from Bacillus stearothermophilus STB04 (BstMFAse) is a thermostable enzyme that preferentially produces maltopentaose… read more here.

Keywords: maltooligosaccharide forming; structure based; product specificity; specificity ... See more keywords

Unraveling the Origins of Changing Product Specificity Properties of Arginine Methyltransferase PRMT7 by the E181D and E181D/Q329A Mutations through QM/MM MD and Free-Energy Simulations.

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recommendations! Published in 2022 at "Journal of chemical theory and computation"

DOI: 10.1021/acs.jctc.1c01219

Abstract: Arginine methylations can regulate important biological processes and affect many cellular activities, and the enzymes that catalyze the methylations are protein arginine methyltransferases (PRMTs). The biological consequences of arginine methylations depend on the methylation states… read more here.

Keywords: e181d e181d; e181d; product specificity; e181d q329a ... See more keywords

Tetraterpene Synthase Substrate and Product Specificity in the Green Microalga Botryococcus braunii Race L.

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recommendations! Published in 2017 at "ACS chemical biology"

DOI: 10.1021/acschembio.7b00457

Abstract: Recently, the biosynthetic pathway for lycopadiene, a C40 tetraterpenoid hydrocarbon, was deciphered from the L race of Botryococcus braunii, an alga that produces hydrocarbon oils capable of being converted into combustible fuels. The lycopadiene pathway… read more here.

Keywords: product specificity; synthase; los; substrate product ... See more keywords

Change of the Product Specificity of a Cyclodextrin Glucanotransferase by Semi-Rational Mutagenesis to Synthesize Large-Ring Cyclodextrins

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recommendations! Published in 2019 at "Catalysts"

DOI: 10.3390/catal9030242

Abstract: Cyclodextrin glucanotransferases (CGTases) convert starch to cyclodextrins (CD) of various sizes. To engineer a CGTase for the synthesis of large-ring CD composed of 9 to 12 glucose units, a loop structure of the protein involved… read more here.

Keywords: rational mutagenesis; semi rational; product; product specificity ... See more keywords

Identification of a Thermostable Levansucrase from Pseudomonas orientalis That Allows Unique Product Specificity at Different Temperatures

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recommendations! Published in 2023 at "Polymers"

DOI: 10.3390/polym15061435

Abstract: The biological production of levan by levansucrase (LS, EC 2.4.1.10) has aroused great interest in the past few years. Previously, we identified a thermostable levansucrase from Celerinatantimonas diazotrophica (Cedi-LS). A novel thermostable LS from Pseudomonas… read more here.

Keywords: pseudomonas orientalis; hmw levan; levan; thermostable levansucrase ... See more keywords