Production and purification of bacterial membrane vesicles for biotechnology applications: Challenges and opportunities
Sign Up to like & getrecommendations! Published in 2022 at "ELECTROPHORESIS"
DOI: 10.1002/elps.202200133
Abstract: Bacterial membrane vesicles (BMVs) are bi‐layered nanostructures derived from Gram‐negative and Gram‐positive bacteria. Among other pathophysiological roles, BMVs are critical messengers in intercellular communication. As a result, BMVs are emerging as a promising technology for… read more here.
Keywords: purification; membrane vesicles; production; production purification ... See more keywords
Production, Purification, and Titration of First-Generation Adenovirus Vectors.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7231-9_28
Abstract: Vectors based on human adenovirus are highly efficient tools for transient genetic modifications of cells or tissues in vitro and in vivo. They can be utilized for gene addition strategies, knockdown strategies and as transfer… read more here.
Keywords: first generation; adenovirus vectors; adenovirus; titration first ... See more keywords
Scalable Production and Purification of Adeno-Associated Viral Vectors (AAV).
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8730-6_17
Abstract: Here we describe methods for the production of adeno-associated viral (AAV) vectors by transient transfection of HEK293 cells grown in serum-free medium in orbital shaken bioreactors and the subsequent purification of vector particles. The protocol… read more here.
Keywords: scalable production; production; associated viral; adeno associated ... See more keywords
Production and purification of recombinant human SPARC.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2017.08.020
Abstract: The matricellular protein SPARC (secreted protein acidic and rich in cysteine, also known as osteonectin or as BM-40) is a collagen-binding protein with a capacity to induce cell rounding and influence proliferation in cultured cells.… read more here.
Keywords: purification recombinant; recombinant human; sparc; recombinant sparc ... See more keywords
Microbial lipases: An overview of screening, production and purification
Sign Up to like & getrecommendations! Published in 2019 at "Biocatalysis and Agricultural Biotechnology"
DOI: 10.1016/j.bcab.2019.101368
Abstract: Abstract Lipases are industrially important biocatalyst, particularly microbial lipases and therefore, screening, production and purification of lipase enzyme from microbial strains are continuously emerging to fulfil the needs of pharmaceutical and food industries. More recently,… read more here.
Keywords: production; screening production; microbial lipases; microbial strains ... See more keywords
Biodiesel from waste frying oils: Methods of production and purification
Sign Up to like & getrecommendations! Published in 2019 at "Energy Conversion and Management"
DOI: 10.1016/j.enconman.2019.01.061
Abstract: Abstract Waste frying oils (WFO) are a promising source in terms of feedstock for biodiesel production, and have a lower cost than the typical refined vegetable oils. In addition, the use of WFO is an… read more here.
Keywords: biodiesel; waste frying; production purification; production ... See more keywords
Production, purification and biochemical characterization of a novel detergent-stable serine alkaline protease from Bacillus safensis strain RH12.
Sign Up to like & getrecommendations! Published in 2019 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2018.10.139
Abstract: A novel extracellular protease (SAPRH) was hyper-produced (9000 U/mL) from Bacillus safensis RH12, a newly isolated enzyme from a Tunisian offshore oil field. The enzyme was purified to homogeneity, using salt-precipitation, heat-treatment and FPLC anion-exchange chromatography.… read more here.
Keywords: bacillus safensis; detergent; production purification; protease ... See more keywords
Enhanced production, purification and biochemical characterization of therapeutic potential fibrinolytic enzyme from a new Bacillus flexus from marine environment
Sign Up to like & getrecommendations! Published in 2020 at "Journal of King Saud University - Science"
DOI: 10.1016/j.jksus.2020.09.004
Abstract: Abstract Objectives The main aim of this study is to isolate and characterize fibrinolytic enzyme from Bacillus flexus. Methods Fish meal of Sardinella longiceps and anchovy was optimized using a two-level full factorial design (25)… read more here.
Keywords: fibrinolytic enzyme; enzyme; bacillus flexus; enhanced production ... See more keywords
Production, Purification and Characterization of a Novel Bacteriocin Produced by Bacillus subtilis VS Isolated from Mango (Mangifera indica L.)
Sign Up to like & getrecommendations! Published in 2021 at "Brazilian Archives of Biology and Technology"
DOI: 10.1590/1678-4324-2021190749
Abstract: HIGHLIGHT Bacillus subtilis VS, an isolate from mango with bacteriocin producing ability. Bacteriocin potentially inhibited pathogenic S. boydii, S. typhi & amp; L. monocytogenes. DEAE cellulose and sephadex G75 chromatography are best suited for purification.… read more here.
Keywords: characterization novel; bacteriocin; bacillus subtilis; purification characterization ... See more keywords
Production and Purification of Pectinase from Bacillus subtilis 15A-B92 and Its Biotechnological Applications
Sign Up to like & getrecommendations! Published in 2022 at "Molecules"
DOI: 10.3390/molecules27134195
Abstract: Enzymes that degrade pectin are called pectinases. Pectinases of microbial origin are used in juice clarification as the process is cost-effective. This study screened a pectinase-producing bacterium isolated from soil and identified as Bacillus subtilis… read more here.
Keywords: pectinase; bacillus subtilis; purification pectinase; subtilis 15a ... See more keywords
ZIKV Envelope Domain-Specific Antibodies: Production, Purification and Characterization
Sign Up to like & getrecommendations! Published in 2019 at "Viruses"
DOI: 10.3390/v11080748
Abstract: Infection with Zika virus (ZIKV) came first to public attention after it was found to be associated with congenital microcephaly during the outbreak in Brazil (2015–2016). Diagnosis of ZIKV suffers from extensive cross-reactivity with other… read more here.
Keywords: purification characterization; zikv envelope; production purification; zikv ... See more keywords