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Published in 2023 at "Applied and Environmental Microbiology"
DOI: 10.1128/aem.00013-23
Abstract: This study developed a three-enzyme cascade pathway for the production of pyrrolidone from l-Glu. The catalytic efficiency of carnitine CoA ligase from Escherichia coli (EcCaiC) was improved by mechanism-based protein engineering, and the titer of…
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Keywords:
enzyme cascade;
pyrrolidone glutamate;
escherichia coli;
pyrrolidone ... See more keywords