Production of Recombinant Factor VIII in Human Cell Lines.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7312-5_5
Abstract: Human cell lines can produce recombinant proteins much more similar to their natural counterpart, compared to other mammalian cell lines, reducing potential immunogenic reactions. Recombinant proteins produced in nonhuman cells can have in its structure… read more here.
Keywords: cell lines; recombinant factor; human cell; cell ... See more keywords
Production of Recombinant Trypanosoma cruzi Antigens in Leishmania tarentolae.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9148-8_8
Abstract: Trypanosomatids are unicellular organisms that colonize a wide diversity of environments and hosts. For instance, Trypanosoma cruzi is a human pathogen responsible for Chagas diseases, while Leishmania tarentolae infects amphibians and became a biotechnological tool… read more here.
Keywords: tarentolae; production recombinant; cruzi; cruzi antigens ... See more keywords
Enhanced production of recombinant proteins by a small molecule protein synthesis enhancer in combination with an antioxidant in recombinant Chinese hamster ovary cells
Sign Up to like & getrecommendations! Published in 2017 at "Bioprocess and Biosystems Engineering"
DOI: 10.1007/s00449-017-1767-1
Abstract: The improvement in the production of recombinant proteins has been linked in a number of small molecules such as carboxylic acids to the inhibition of histone deacetylase, leading to increased transcription of genes. However, carboxylic… read more here.
Keywords: acid; production; butanoic acid; pentanoic acid ... See more keywords
A New Plant Expression System for Producing Pharmaceutical Proteins
Sign Up to like & getrecommendations! Published in 2020 at "Molecular Biotechnology"
DOI: 10.1007/s12033-020-00242-2
Abstract: In the past decade, interest in the production of recombinant pharmaceutical proteins in plants has tremendously progressed because plants do not harbor mammalian viruses, are economically competitive, easily scalable, and capable of carrying out complex… read more here.
Keywords: protein; pharmaceutical proteins; infiltration; production recombinant ... See more keywords
Production of recombinant beta-amylase of Bacillus aryabhattai
Sign Up to like & getrecommendations! Published in 2019 at "Preparative Biochemistry and Biotechnology"
DOI: 10.1080/10826068.2018.1536987
Abstract: Abstract In this study, the effects of carbon source, nitrogen source, and metal ions on cell growth and Bacillus aryabhattai β-amylase production in recombinant Brevibacillus choshinensis were investigated. The optimal medium for β-amylase production, containing… read more here.
Keywords: production; medium; amylase; bacillus aryabhattai ... See more keywords
Production of recombinant coagulation factors: Are humans the best host cells?
Sign Up to like & getrecommendations! Published in 2017 at "Bioengineered"
DOI: 10.1080/21655979.2017.1279767
Abstract: ABSTRACT The main treatment option for Hemophilia A/B patients involves the administration of recombinant coagulation factors on-demand or in a prophylactic approach. Despite the safety and efficacy of this replacement therapy, the development of antibodies… read more here.
Keywords: recombinant coagulation; coagulation factors; production recombinant; human cell ... See more keywords
Development of a new promoter to avoid the silencing of genes in the production of recombinant antibodies in chinese hamster ovary cells
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Biological Engineering"
DOI: 10.1186/s13036-019-0187-y
Abstract: BackgroundThe production of recombinant proteins in mammalian cell lines is one of the most important areas in biopharmaceutical industry. Viral transcriptional promoters are widely used to express recombinant proteins in mammalian cell lines. However, these… read more here.
Keywords: production; cell lines; chinese hamster; promoter ... See more keywords
Feasibility Study for Bedside Production of Recombinant Human Acid α-Glucosidase: Technical and Financial Considerations.
Sign Up to like & getrecommendations! Published in 2020 at "Current pharmaceutical biotechnology"
DOI: 10.2174/1389201021666200217113049
Abstract: The high cost of orphan drugs limits their access by many patients, especially in low- and middle-income countries. Many orphan drugs are off-patent without alternative generic or biosimilar versions available. Production of these drugs at… read more here.
Keywords: recombinant human; production; bedside production; human acid ... See more keywords
Glutamate as a non-conventional substrate for high production of the recombinant protein in Escherichia coli
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.991963
Abstract: The economic viability of the biomass-based biorefinery is readily acknowledged by implementation of a cascade process that produces value-added products such as enzymes prior to biofuels. Proteins from the waste stream of biorefinery processes generally… read more here.
Keywords: glutamate non; glu; non conventional; escherichia coli ... See more keywords
Production of Recombinant Active Human TGFβ1 in Nicotiana benthamiana
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2022.922694
Abstract: The production of recombinant proteins in plant systems is receiving wider attention. Indeed, various plant-produced pharmaceuticals have been shown to be biologically active. However, the production of human growth factors and cytokines in heterologous systems… read more here.
Keywords: human tgf; production; nicotiana benthamiana; production recombinant ... See more keywords
Impact of SARS-CoV-2 RBD Mutations on the Production of a Recombinant RBD Fusion Protein in Mammalian Cells
Sign Up to like & getrecommendations! Published in 2022 at "Biomolecules"
DOI: 10.3390/biom12091170
Abstract: SARS-CoV-2 receptor-binding domain (RBD) is a major target for the development of diagnostics, vaccines and therapeutics directed against COVID-19. Important efforts have been dedicated to the rapid and efficient production of recombinant RBD proteins for… read more here.
Keywords: recombinant rbd; production recombinant; sars cov; protein ... See more keywords