Computational modeling highlights the role of the disordered Formin Homology 1 domain in profilin‐actin transfer
Sign Up to like & getrecommendations! Published in 2018 at "FEBS Letters"
DOI: 10.1002/1873-3468.13088
Abstract: Formins accelerate actin polymerization, assumed to occur through flexible Formin Homology 1 (FH1) domain‐mediated transfer of profilin‐actin to the barbed end. To study FH1 properties and address sequence effects, including varying length/distribution of profilin‐binding proline‐rich… read more here.
Keywords: profilin; formin homology; profilin actin; transfer ... See more keywords
Toxoplasma gondii profilin and tachyzoites RH strain may manipulate autophagy via downregulating Atg5 and Atg12 and upregulating Atg7.
Sign Up to like & getrecommendations! Published in 2021 at "Molecular biology reports"
DOI: 10.1007/s11033-021-06667-5
Abstract: BACKGROUND Autophagy process is an important defense mechanism against intracellular infection. This process plays a critical role in limiting the development of Toxoplasma gondii. This study aimed to investigate the effects of T. gondii profilin… read more here.
Keywords: profilin tachyzoites; gondii profilin; fold value; profilin ... See more keywords
Characterization of Profilin Binding Kinetics using Ensemble Molecular Dynamics Simulations
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.1554
Abstract: We investigate the dynamics of profilin binding, including differences in binding for the loading and recruiting subregions of VASP, the effect of binding site mutations on peptide affinity for profilin, and the effect of actin… read more here.
Keywords: ensemble molecular; profilin; molecular dynamics; profilin binding ... See more keywords
Biphasic Effect of Profilin Impacts the Formin mDia1 Force-Sensing Mechanism in Actin Polymerization.
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical journal"
DOI: 10.1016/j.bpj.2017.06.012
Abstract: Formins are force-sensing proteins that regulate actin polymerization dynamics. Here, we applied stretching tension to individual actin filaments under the regulation of formin mDia1 to investigate the mechanical responses in actin polymerization dynamics. We found… read more here.
Keywords: profilin; actin; actin polymerization; effect ... See more keywords
Actin Cytoskeleton: Profilin Gives Cells an Edge
Sign Up to like & getrecommendations! Published in 2020 at "Current Biology"
DOI: 10.1016/j.cub.2020.05.041
Abstract: A new study reports that the actin-monomer-binding protein profilin 1 dictates protrusion character at the cell edge. These findings help explain how distinct, tunable actin polymerization pathways collaborate to form higher-order cellular structures. read more here.
Keywords: actin cytoskeleton; profilin; edge; profilin gives ... See more keywords
Synergistic effect of GRA7 and profilin proteins in vaccination against chronic Toxoplasma gondii infection.
Sign Up to like & getrecommendations! Published in 2021 at "Vaccine"
DOI: 10.1016/j.vaccine.2020.12.072
Abstract: Toxoplasmosis is a zoonotic disease with worldwide prevalence in humans and warm-blooded animal populations. In livestock Toxoplasma gondii is the causal agent of significant economic losses since it can cause abortions in goats and sheep.… read more here.
Keywords: gondii infection; profilin; gra7; gondii ... See more keywords
Self-Assembly of Human Profilin-1 Detected by Carr-Purcell-Meiboom-Gill Nuclear Magnetic Resonance (CPMG NMR) Spectroscopy.
Sign Up to like & getrecommendations! Published in 2017 at "Biochemistry"
DOI: 10.1021/acs.biochem.6b01263
Abstract: Protein oligomerization in the cell has important implications for both health and disease, and an understanding of the mechanisms by which proteins can self-associate is, therefore, of critical interest. Initial stages of the oligomerization process… read more here.
Keywords: nuclear magnetic; profilin; human profilin; magnetic resonance ... See more keywords
Global ubiquitinome profiling identifies NEDD4 as a regulator of Profilin 1 and actin remodelling in neural crest cells.
Sign Up to like & getrecommendations! Published in 2022 at "Nature communications"
DOI: 10.1038/s41467-022-29660-3
Abstract: The ubiquitin ligase NEDD4 promotes neural crest cell (NCC) survival and stem-cell like properties to regulate craniofacial and peripheral nervous system development. However, how ubiquitination and NEDD4 control NCC development remains unknown. Here we combine… read more here.
Keywords: ubiquitinome profiling; profilin; global ubiquitinome; crest ... See more keywords
Visualizing molecules of functional human profilin
Sign Up to like & getrecommendations! Published in 2022 at "eLife"
DOI: 10.1101/2021.09.01.458557
Abstract: Profilin-1 (PFN1) is a cytoskeletal protein that regulates the dynamics of actin and microtubule assembly. Thus, PFN1 is essential for the normal division, motility, and morphology of cells. Unfortunately, conventional fusion and direct labeling strategies… read more here.
Keywords: molecules functional; biochemical assays; pfn1; visualizing molecules ... See more keywords
A function of profilin in force generation during malaria parasite motility independent of actin binding.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of cell science"
DOI: 10.1242/jcs.233775
Abstract: During transmission of malaria-causing parasites from mosquito to mammal, Plasmodium sporozoites migrate at high speed within the skin to access the bloodstream and infect the liver. This unusual gliding motility is based on retrograde flow… read more here.
Keywords: profilin; force generation; actin binding; force ... See more keywords
A unique profilin-actin interface is important for malaria parasite motility
Sign Up to like & getrecommendations! Published in 2017 at "PLoS Pathogens"
DOI: 10.1371/journal.ppat.1006412
Abstract: Profilin is an actin monomer binding protein that provides ATP-actin for incorporation into actin filaments. In contrast to higher eukaryotic cells with their large filamentous actin structures, apicomplexan parasites typically contain only short and highly… read more here.
Keywords: malaria parasite; profilin; actin; profilin actin ... See more keywords