Rapid and Quantitative Profiling of Substrate Specificity of ω‐Transaminases for Ketones
Sign Up to like & getrecommendations! Published in 2019 at "ChemCatChem"
DOI: 10.1002/cctc.201900399
Abstract: ω‐Transaminases (ω‐TAs) have gained growing attention owing to their capability for asymmetric synthesis of chiral amines from ketones. Reliable high‐throughput activity assay of ω‐TAs is essential in carrying out extensive substrate profiling and establishing a… read more here.
Keywords: profiling; quantitative profiling; profiling substrate; substrate profiling ... See more keywords
Mass Spectrometry-Based Profiling of Plant Sphingolipids from Typical and Aberrant Metabolism.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1362-7_10
Abstract: Mass spectrometry has increasingly been used as a tool to complement studies of sphingolipid metabolism and biological functions in plants and other eukaryotes. Mass spectrometry is now essential for comprehensive sphingolipid analytical profiling because of… read more here.
Keywords: profiling; spectrometry; mass spectrometry; metabolism ... See more keywords
A disease-driver population within interstitial cells of human calcific aortic valves identified via single-cell and proteomic profiling.
Sign Up to like & getrecommendations! Published in 2022 at "Cell reports"
DOI: 10.1016/j.celrep.2022.110685
Abstract: Cellular heterogeneity of aortic valves complicates the mechanistic evaluation of the calcification processes in calcific aortic valve disease (CAVD), and animal disease models are lacking. In this study, we identify a disease-driver population (DDP) within… read more here.
Keywords: calcific aortic; profiling; cell; disease ... See more keywords
Longitudinal profiling of respiratory and systemic immune responses reveals myeloid cell-driven lung inflammation in severe COVID-19
Sign Up to like & getrecommendations! Published in 2021 at "Immunity"
DOI: 10.1016/j.immuni.2021.03.005
Abstract: Immune response dynamics in COVID-19 and its severe manifestations have largely been studied in circulation. Here, we examined the relationship between immune processes in the respiratory tract and circulation through longitudinal phenotypic, transcriptomic and cytokine… read more here.
Keywords: immune; profiling; inflammation; respiratory ... See more keywords
Metabolomic profiling reveals plasma GlycA and GlycB as a potential biomarkers for treatment efficiency in rheumatoid arthritis.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of pharmaceutical and biomedical analysis"
DOI: 10.1016/j.jpba.2021.113971
Abstract: In this pilot study, we carried out metabolic profiling of patients with rheumatoid arthritis (RA) starting therapy with biological disease-modifying antirheumatic drugs (bDMARDs). The main aim of the study was to assess the occurring metabolic… read more here.
Keywords: profiling; treatment; glyca glycb; non responders ... See more keywords
Are all ‘ultra-processed’ foods nutritional demons? A commentary and nutritional profiling analysis.
Sign Up to like & getrecommendations! Published in 2019 at "Trends in Food Science and Technology"
DOI: 10.1016/j.tifs.2019.08.023
Abstract: Abstract Background There has been a growing body of literature published on ultra-processed foods (UPF). This has been driven by an ambiguous definition that can be easily applied to observational work. Nutritionally, UPF have been… read more here.
Keywords: profiling; analysis; percent; ultra processed ... See more keywords
Pharmacologic profiling reveals lapatinib as a novel antiviral against SARS-CoV-2 in vitro
Sign Up to like & getrecommendations! Published in 2021 at "Virology"
DOI: 10.1016/j.virol.2021.11.008
Abstract: The emergence of SARS-CoV-2 virus has resulted in a worldwide pandemic, but effective antiviral therapies are not widely available. To improve treatment options, we conducted a high-throughput screen to uncover compounds that block SARS-CoV-2 infection.… read more here.
Keywords: profiling; sars cov; pharmacologic profiling; reveals lapatinib ... See more keywords
A Rapid Array-Based Approach to N-Glycan Profiling of Cultured Cells.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.9b00303
Abstract: Typically, N-glycosylation studies done on cultured cells require up to millions of cells followed by lengthy preparation to release, isolate, and profile N-glycans. To overcome these limitations, we report a rapid array-based workflow for profiling… read more here.
Keywords: profiling; glycan profiling; cultured cells; approach glycan ... See more keywords
Chemoproteomic Profiling of Protein Substrates of a Major Lysine Acetyltransferase in the Native Cellular Context.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.1c00935
Abstract: The family of lysine acetyltransferases (KATs) regulates epigenetics and signaling pathways in eukaryotic cells. So far, knowledge of different KAT members contributing to the cellular acetylome is limited, which limits our understanding of biological functions… read more here.
Keywords: coa; acetyltransferase; protein substrates; profiling ... See more keywords
Microbial abundance, activity and population genomic profiling with mOTUs2
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-08844-4
Abstract: Metagenomic sequencing has greatly improved our ability to profile the composition of environmental and host-associated microbial communities. However, the dependency of most methods on reference genomes, which are currently unavailable for a substantial fraction of… read more here.
Keywords: profiling; microbial abundance; activity population; abundance ... See more keywords
Genetic and Epigenetic Profiling Reveals EZH2-mediated Down Regulation of OCT-4 Involves NR2F2 during Cardiac Differentiation of Human Embryonic Stem Cells
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-13442-9
Abstract: Human embryonic (hES) stem cells are widely used as an in vitro model to understand global genetic and epigenetic changes that occur during early embryonic development. In-house derived hES cells (KIND1) were subjected to directed… read more here.
Keywords: profiling; differentiation; genetic epigenetic; stem cells ... See more keywords