Profiling Protein-DNA Interactions Cell-Type-Specifically with Targeted DamID.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2140-0_11
Abstract: Targeted DamID (TaDa) is a means of profiling the binding of any DNA-associated protein cell-type specifically, including transcription factors, RNA polymerase, and chromatin-modifying proteins. The technique is highly sensitive, highly reproducible, requires no mechanical disruption,… read more here.
Keywords: dna; profiling protein; targeted damid; cell type ... See more keywords
WaterKit: Thermodynamic Profiling of Protein Hydration Sites.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of chemical theory and computation"
DOI: 10.1021/acs.jctc.2c01087
Abstract: Water desolvation is one of the key components of the free energy of binding of small molecules to their receptors. Thus, understanding the energetic balance of solvation and desolvation resulting from individual water molecules can… read more here.
Keywords: waterkit thermodynamic; protein hydration; water; water molecules ... See more keywords
Biologically Derived Neoproteoglycans for Profiling Protein-Glycosaminoglycan Interactions.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.2c00205
Abstract: Glycosaminoglycans (GAGs) are a class of highly negatively charged membrane-associated and extracellular matrix polysaccharides involved in the regulation of myriad biological functions, including cell adhesion, migration, signaling, and differentiation, among others. GAGs are typically attached… read more here.
Keywords: gag; derived neoproteoglycans; binding proteins; profiling protein ... See more keywords
Bioorthogonal Reporters for Detecting and Profiling Protein Acetylation and Acylation
Sign Up to like & getrecommendations! Published in 2019 at "SLAS Discovery"
DOI: 10.1177/2472555219887144
Abstract: Protein acylation, exemplified by lysine acetylation, is a type of indispensable and widespread protein posttranslational modification in eukaryotes. Functional annotation of various lysine acetyltransferases (KATs) is critical to understanding their regulatory roles in abundant biological… read more here.
Keywords: protein acetylation; acetylation; acylation; biology ... See more keywords