Transcriptome-Wide Profiling of RNA Stability.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1851-6_17
Abstract: Gene expression is controlled at multiple levels, including RNA transcription and turnover. But determining the relative contributions of RNA biogenesis and decay to the steady-state abundance of cellular transcripts remains challenging because conventional transcriptomics approaches… read more here.
Keywords: profiling rna; rna stability; transcriptome wide; wide profiling ... See more keywords
Robust, Cost-Effective Profiling of RNA Binding Protein Targets with Single-end Enhanced Crosslinking and Immunoprecipitation (seCLIP).
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7204-3_14
Abstract: Profiling of RNA binding protein targets in vivo provides critical insights into the mechanistic roles they play in regulating RNA processing. The enhanced crosslinking and immunoprecipitation (eCLIP) methodology provides a framework for robust, reproducible identification… read more here.
Keywords: profiling rna; methodology; end; rna ... See more keywords
Antigen receptor repertoire profiling from RNA-seq data
Sign Up to like & getrecommendations! Published in 2017 at "Nature Biotechnology"
DOI: 10.1038/nbt.3979
Abstract: Somatic recombination and accumulation of mutations in V-D-J segments result in vast heterogeneity of T-cell receptor (TCR) and immunoglobulin repertoires1,2. High-throughput profiling of immune receptors has become an important tool for studies of adaptive immunity… read more here.
Keywords: profiling rna; repertoire profiling; rna seq; receptor ... See more keywords
Global profiling of RNA-binding protein target sites by LACE-seq.
Sign Up to like & getrecommendations! Published in 2021 at "Nature cell biology"
DOI: 10.1038/s41556-021-00696-9
Abstract: RNA-binding proteins (RBPs) have essential functions during germline and early embryo development. However, current methods are unable to identify the in vivo targets of a RBP in these low-abundance cells. Here, by coupling RBP-mediated reverse… read more here.
Keywords: global profiling; profiling rna; rna binding; lace seq ... See more keywords