Programmable RNA manipulation in living cells
Sign Up to like & getrecommendations! Published in 2019 at "Cellular and Molecular Life Sciences"
DOI: 10.1007/s00018-019-03252-9
Abstract: RNAs are responsible for mediating genetic information flow within the cell. RNA splicing, modification, trafficking, translation, and stability are all controlled at the transcript level. However, biological tools to study and manipulate them in a… read more here.
Keywords: rna manipulation; manipulation living; living cells; manipulation ... See more keywords
In Vivo Repair of a Protein Underlying a Neurological Disorder by Programmable RNA Editing
Sign Up to like & getrecommendations! Published in 2020 at "Cell reports"
DOI: 10.1016/j.celrep.2020.107878
Abstract: SUMMARY Programmable RNA editing is gaining momentum as an approach to repair mutations, but its efficiency in repairing endogenous mutant RNA in complex tissue is unknown. Here we apply this approach to the brain and… read more here.
Keywords: repair protein; vivo repair; rna; rna editing ... See more keywords
Programmable RNA editing with compact CRISPR–Cas13 systems from uncultivated microbes
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DOI: 10.1038/s41592-021-01124-4
Abstract: Competitive coevolution between microbes and viruses has led to the diversification of CRISPR–Cas defense systems against infectious agents. By analyzing metagenomic terabase datasets, we identified two compact families (775 to 803 amino acids (aa)) of… read more here.
Keywords: rna base; rna editing; cas13; programmable rna ... See more keywords
The Basis and Promise of Programmable RNA Editing and Modification
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Genetics"
DOI: 10.3389/fgene.2022.834413
Abstract: One key advantage of RNA over genomic editing is its temporary effects. Aside from current use of DNA-targeting CRISPR-Cas9, the more recently discovered CRISPR-Cas13 has been explored as a means of editing due to its… read more here.
Keywords: rna editing; crispr; rna; programmable rna ... See more keywords