Progress Curve Analysis of the One Stage Chromogenic Assay for Ecarin.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2020.113907
Abstract: Snake venom prothrombin activators such as Ecarin are readily assayed by continuous spectrophotometric monitoring of p-nitroaniline production in a one step assay containing prothrombin and a p-nitroanilide peptide substrate for thrombin. The coupled reactions result… read more here.
Keywords: progress curve; progress; analysis one; analysis ... See more keywords
In vitro reconstitution of kallikrein-kinin system and progress curve analysis
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DOI: 10.1042/bsr20221081
Abstract: Abstract Human kallikrein-kinin system (KKS) is a proteolytic cascade with two serine-protease zymogen couples (Factor XII and prekallikrein (PK) and their activated forms, FXIIa, PKa, respectively), releasing bradykinin by cleavage of native high-molecular-weight kininogen (nHK)… read more here.
Keywords: progress curve; curve analysis; curve; kallikrein kinin ... See more keywords
Abstract 2766: Automation of linear range determination: Enzymatic progress curve applications
Sign Up to like & getrecommendations! Published in 2023 at "Cancer Research"
DOI: 10.1158/1538-7445.am2023-2766
Abstract: To characterize modulators of enzymatic activity, a continuous assay format will quantify with confidence the initial reaction velocity far better than an end point assay. When the progress curve is linear from the start and… read more here.
Keywords: progress curve; curve; range; determination ... See more keywords