Diabetes-Associated Mutations in Proinsulin Provide a “Molecular Rheostat” of Nascent Foldability
Sign Up to like & getrecommendations! Published in 2022 at "Current Diabetes Reports"
DOI: 10.1007/s11892-022-01447-2
Abstract: Purpose of Review Diabetes mellitus (DM) due to toxic misfolding of proinsulin variants provides a monogenic model of endoplasmic reticulum (ER) stress. The mutant proinsulin syndrome (also designated MIDY; Mutant INS -gene-induced Diabetes of Youth… read more here.
Keywords: foldability; proinsulin; cell; midy mutations ... See more keywords
The ketogenic diet corrects metabolic hypogonadism and preserves pancreatic ß-cell function in overweight/obese men: a single-arm uncontrolled study
Sign Up to like & getrecommendations! Published in 2020 at "Endocrine"
DOI: 10.1007/s12020-020-02518-8
Abstract: Overweight and obesity are increasingly spread in our society. Low testosterone levels are often present in these patients, the so-called metabolic hypogonadism, that further alters the metabolic balance in a sort of vicious cycle. Very… read more here.
Keywords: insulin; overweight obese; metabolic hypogonadism; proinsulin ... See more keywords
Identification of a novel proinsulin-associated SNP and demonstration that proinsulin is unlikely to be a causal factor in subclinical vascular remodelling using Mendelian randomisation
Sign Up to like & getrecommendations! Published in 2017 at "Atherosclerosis"
DOI: 10.1016/j.atherosclerosis.2017.09.031
Abstract: Background and aims Increased proinsulin relative to insulin levels have been associated with subclinical atherosclerosis (measured by carotid intima-media thickness (cIMT)) and are predictive of future cardiovascular disease (CVD), independently of established risk factors. The… read more here.
Keywords: vascular remodelling; subclinical vascular; associated snp; mendelian randomisation ... See more keywords
UGGT1 retains proinsulin in the endoplasmic reticulum in an arginine dependent manner.
Sign Up to like & getrecommendations! Published in 2020 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2020.04.158
Abstract: We sought to clarify a pathway by which L- and dD-arginine simulate insulin secretion in mice and cell lines and obtained the following novel two findings. (1) Using affinity magnetic nanobeads technology, we identified that… read more here.
Keywords: endoplasmic reticulum; insulin secretion; uggt1 retains; arginine ... See more keywords
A luciferase immunoprecipitation assay for the detection of proinsulin/insulin autoantibodies.
Sign Up to like & getrecommendations! Published in 2018 at "Clinical biochemistry"
DOI: 10.1016/j.clinbiochem.2018.02.011
Abstract: AIM Luciferase immunoprecipitation (LIPS) assays show good sensitivity and specificity in testing islet specific autoantibodies for diagnosis of type 1 diabetes (T1D). However, there are currently no LIPS assays available for detecting proinsulin/insulin autoantibody (IAA)… read more here.
Keywords: insulin; luciferase immunoprecipitation; proinsulin; fusion ... See more keywords
Persistence of Pancreatic Insulin mRNA Expression and Proinsulin Protein in Type 1 Diabetes Pancreata.
Sign Up to like & getrecommendations! Published in 2017 at "Cell metabolism"
DOI: 10.1016/j.cmet.2017.08.013
Abstract: The canonical notion that type 1 diabetes (T1D) results following a complete destruction of β cells has recently been questioned as small amounts of C-peptide are detectable in patients with long-standing disease. We analyzed protein… read more here.
Keywords: insulin; type diabetes; proinsulin; expression ... See more keywords
Microbiota guides insulin trafficking in beta cells
Sign Up to like & getrecommendations! Published in 2019 at "Cell Research"
DOI: 10.1038/s41422-019-0200-5
Abstract: Pancreatic beta cells produce insulin on intricate cellular assembly line, but the extent of the gut microbiota involvement was not clear. It emerges that fragments of bacterial cell wall released by lysozyme 1 in the… read more here.
Keywords: insulin; beta cells; proinsulin; insulin vesicles ... See more keywords
Morphologically ‘invisible’ proinsulin – secreting adenoma detected by Ga‐68 Exendin‐4 (GLP‐1 Receptor) positron emission tomography/CT
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Medical Imaging and Radiation Oncology"
DOI: 10.1111/1754-9485.12704
Abstract: This case report of a young man suffering from recurring hypoglycaemia illustrates a rare condition of a neuroendocrine tumour, predominantly secreting proinsulin and invisible to conventional imaging approaches. Only a GLP‐1 receptor PET/CT using Exendin‐4… read more here.
Keywords: morphologically invisible; proinsulin secreting; proinsulin; invisible proinsulin ... See more keywords
Insulin null ß-cells have a prohormone processing defect that is not reversed by AAV rescue of proinsulin expression.
Sign Up to like & getrecommendations! Published in 2022 at "Endocrinology"
DOI: 10.1210/endocr/bqac051
Abstract: Up to 6% of diabetes has a monogenic cause including mutations in the insulin gene and patients are candidates for a gene therapy. Using a mouse model of permanent neonatal diabetes, we assessed the efficacy… read more here.
Keywords: ins1; processing defect; expression; proinsulin ... See more keywords
A Novel Somatic Mutation Implicates ATP6V0D1 in Proinsulin Processing
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the Endocrine Society"
DOI: 10.1210/jendso/bvac196
Abstract: Abstract Context Prohormone convertase 1/3 (PC1/3), encoded by protein convertase subtilisin kexin type 1 (PCSK1), converts inactive prohormones into biologically active peptides. Somatic mutations of insulinomas are associated with genetic defects interfering with control of… read more here.
Keywords: somatic mutation; proinsulin processing; proinsulin levels; insulin peptide ... See more keywords
ERp29 as a regulator of Insulin biosynthesis
Sign Up to like & getrecommendations! Published in 2020 at "PLoS ONE"
DOI: 10.1371/journal.pone.0233502
Abstract: The environment within the Endoplasmic Reticulum (ER) influences Insulin biogenesis. In particular, ER stress may contribute to the development of Type 2 Diabetes (T2D) and Cystic Fibrosis Related Diabetes (CFRD), where evidence of impaired Insulin… read more here.
Keywords: erp29; insulin; whole cell; insulin biosynthesis ... See more keywords