Construction of high level prokaryotic expression and purification system of PD-L1 extracellular domain by using Escherichia coli host cell machinery.
Sign Up to like & getrecommendations! Published in 2017 at "Immunology letters"
DOI: 10.1016/j.imlet.2017.06.004
Abstract: Programmed cell death 1 ligand 1 (PD-L1) is a trans-membrane protein highly expressed on the membrane of cancer cell, which binds inhibitory receptor of PD-1 on the T cells and attenuates anti-tumor immune response.The strategy… read more here.
Keywords: system; escherichia coli; prokaryotic expression; extracellular domain ... See more keywords
Construction and preliminary identification of a prokaryotic expression single-chain antibody fragments library against Streptococcus pneumoniae from antibody-producing cells in human tonsil
Sign Up to like & getrecommendations! Published in 2019 at "Bioengineered"
DOI: 10.1080/21655979.2019.1616492
Abstract: ABSTRACT Tonsillitis is the inflammation of the tonsils due to infection, many patients ultimately have to undergo tonsillectomy. In order to improve the accuracy of diagnosis and even create a new treatment for tonsillitis, we… read more here.
Keywords: chain; chain antibody; pneumoniae; antibody ... See more keywords
Cloning and prokaryotic expression of WRKY48 from Caragana intermedia
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DOI: 10.1515/biol-2022-0016
Abstract: Abstract Caragana intermedia (C. intermedia) is a kind of drought-tolerant leguminous shrub. WRKY transcription factors are one of the largest family of transcription factors in plants and play critical regulatory roles in stress tolerance and… read more here.
Keywords: cloning prokaryotic; expression; caragana intermedia; prokaryotic expression ... See more keywords
Construction and expression of a prokaryotic expression vector for the goat sry gene
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DOI: 10.18805/ijar.b-963
Abstract: Goats are economically important animals in the world, and their sex is an important factor in their economic efficiency. Reconstruction of a goat SRY gene expression vector can lay a foundation for studying the immunogenicity… read more here.
Keywords: expression; sry; expression vector; gene ... See more keywords
Prokaryotic expression and immunogenicity in rabbit of Ebola virus VP40 matrix protein
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DOI: 10.18805/ijar.v0iof.8477
Abstract: Ebola hemorrhagic fever is among severe and fatal viral hemorrhagic fevers and a global health concern. Here, we constructed a prokaryotic expression plasmid pET32a-VP40 for the large-scale expression of VP40 in E. coli Rosetta (DE3)… read more here.
Keywords: vp40 protein; prokaryotic expression; expression; rabbit ... See more keywords