The prolyl hydroxylase OGFOD1 promotes cancer cell proliferation by regulating the expression of cell cycle regulators
Sign Up to like & getrecommendations! Published in 2022 at "FEBS Letters"
DOI: 10.1002/1873-3468.14547
Abstract: OGFOD1, a prolyl‐hydroxylase, has been reported to translocate from the nucleus to the cytoplasm in response to cellular stress. Here, we demonstrate that OGFOD1 regulates the transcription and post‐transcriptional stabilization of cell cycle‐related genes. OGFOD1… read more here.
Keywords: cell; prolyl hydroxylase; cell cycle; cycle regulators ... See more keywords
CRISPR/Cas9-mediated knockout of a prolyl-4-hydroxylase subfamily in Nicotiana benthamiana using DsRed2 for plant selection.
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology journal"
DOI: 10.1002/biot.202100698
Abstract: The properties of host plants used for molecular farming can be modified by CRISPR/Cas9 genome editing to improve the quality and yield of recombinant proteins. However, it is often necessary to target multiple genes simultaneously,… read more here.
Keywords: nicotiana benthamiana; prolyl hydroxylase; crispr cas9; plant ... See more keywords
HIF prolyl hydroxylase 2/3 deletion disrupts astrocytic integrity and exacerbates neuroinflammation.
Sign Up to like & getrecommendations! Published in 2023 at "Glia"
DOI: 10.1002/glia.24380
Abstract: Astrocytes constitute the parenchymal border of the blood-brain barrier (BBB), modulate the exchange of soluble and cellular elements, and are essential for neuronal metabolic support. Thus, astrocytes critically influence neuronal network integrity. In hypoxia, astrocytes… read more here.
Keywords: integrity; prolyl hydroxylase; hydroxylase deletion; hif prolyl ... See more keywords
The prolyl hydroxylase inhibitor GSK1120360A reduces early brain injury, but protection is not maintained in a neonatal rat model of hypoxic ischaemic encephalopathy
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Developmental Neuroscience"
DOI: 10.1002/jdn.10199
Abstract: Hypoxic–ischemic encephalopathy (HIE) in newborns is associated with high morbidity and mortality, with many babies suffering long‐term neurological deficits. Currently, treatment options are limited to therapeutic hypothermia, which is not appropriate for use in all… read more here.
Keywords: prolyl hydroxylase; group; brain injury; gsk1120360a ... See more keywords
Purification and Characterization of Prolyl Hydroxylase 3/Pyruvate Kinase Isoform 2 Protein Complex
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Biotechnology"
DOI: 10.1007/s12033-019-00228-9
Abstract: The prolyl hydroxylase 3 (PHD3) protein is less abundant in normal oxygen conditions (normoxia) but increases under deficient oxygen condition (hypoxia). Since cancerous cells often thrive in hypoxic conditions and predominantly express the Pyruvate kinase… read more here.
Keywords: protein; pyruvate kinase; phd3 pkm2; pkm2 ... See more keywords
Production of functional recombinant prolyl hydroxylase-2 enzyme in insect cells for small molecule inhibitor screening studies.
Sign Up to like & getrecommendations! Published in 2022 at "Protein expression and purification"
DOI: 10.1016/j.pep.2022.106073
Abstract: Prolyl hydroxylase-2 (PHD2) is a dioxygenase enzyme that specifically hydroxylates the hypoxia inducible factor (HIF) which then targets it for degradation in oxygenated cells. Inhibition of the activity of the PHD2 enzyme under hypoxic environmental… read more here.
Keywords: enzyme; prolyl hydroxylase; phd2; inhibitor ... See more keywords
Prolyl 4-Hydroxylase: Substrate Isosteres in Which an (E)- or (Z)-Alkene Replaces the Prolyl Peptide Bond.
Sign Up to like & getrecommendations! Published in 2017 at "Biochemistry"
DOI: 10.1021/acs.biochem.6b00976
Abstract: Collagen prolyl 4-hydroxylases (CP4Hs) catalyze a prevalent posttranslational modification, the hydroxylation of (2S)-proline residues in protocollagen strands. The ensuing (2S,4R)-4-hydroxyproline residues are necessary for the conformational stability of the collagen triple helix. Prolyl peptide bonds… read more here.
Keywords: prolyl; prolyl peptide; prolyl hydroxylase; peptide bond ... See more keywords
Collagen Prolyl 4-Hydroxylase as a Therapeutic Target.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of medicinal chemistry"
DOI: 10.1021/acs.jmedchem.8b00822
Abstract: Collagen is the dominant protein of the extracellular matrix. Its distinguishing feature is a three-stranded helix of great tensile strength. (2 S,4 R)-4-Hydroxyproline residues are essential for the stability of this triple helix. These residues… read more here.
Keywords: collagen; collagen prolyl; chemistry; prolyl hydroxylase ... See more keywords
Chemical activation of prolyl hydroxylase-2 by BBAP-1 down regulates hypoxia inducible factor-1α and fatty acid synthase for mammary gland chemoprevention
Sign Up to like & getrecommendations! Published in 2018 at "RSC Advances"
DOI: 10.1039/c8ra01239c
Abstract: (4-[7-(Acetyloxy)-2-ethyl-2H-chromen-3-yl] phenyl acetate) (BBAP-1) was identified as a potential prolyl hydroxylase-2 activator and tested for this activity using the 2-oxoglutarate dependent in vitro assay. BBAP-1 was evaluated for its cytotoxic potential against ER + MCF-7… read more here.
Keywords: prolyl hydroxylase; bbap; mammary gland;
Inhibition of HIF prolyl hydroxylase modulates platelet function.
Sign Up to like & getrecommendations! Published in 2022 at "Thrombosis and haemostasis"
DOI: 10.1055/a-1837-7797
Abstract: Hypoxia-inducible factors-1α (HIF-1α) involves in redox reaction. Considering the role of reactive oxygen species (ROS) in platelet function, whether it regulates platelet function remains unclear. Using an inhibitor of HIF prolyl hydroxylase IOX-2, we intend… read more here.
Keywords: function; prolyl hydroxylase; hif prolyl; hif ... See more keywords
Skp1 isoforms are differentially modified by a dual function prolyl 4-hydroxylase/N-acetylglucosaminyltransferase in a plant pathogen.
Sign Up to like & getrecommendations! Published in 2019 at "Glycobiology"
DOI: 10.1093/glycob/cwz049
Abstract: Skp1 is hydroxylated by an O2-dependent prolyl hydroxylase (PhyA) that contributes to O2-sensing in the social amoeba Dictyostelium and the mammalian pathogen Toxoplasma gondii. HO-Skp1 is subject to glycosylation and the resulting pentasaccharide affects Skp1… read more here.
Keywords: pathogen; skp1 isoforms; plant pathogen; skp1 ... See more keywords