Learning Context-Dependent DNA Mutation Patterns in Error-Prone Polymerase Chain Reaction.
Sign Up to like & getrecommendations! Published in 2022 at "Biochemistry"
DOI: 10.1021/acs.biochem.2c00292
Abstract: We present a novel statistical learning method for studying context-dependent error rates in error-prone polymerase chain reaction (PCR) experiments. We demonstrate the method by applying it to error-prone PCR sequencing data and show how it… read more here.
Keywords: chain reaction; polymerase chain; error prone; context dependent ... See more keywords
The Error-Prone Polymerase DnaE2 Mediates the Evolution of Antibiotic Resistance in Persister Mycobacterial Cells
Sign Up to like & getrecommendations! Published in 2022 at "Antimicrobial Agents and Chemotherapy"
DOI: 10.1128/aac.01773-21
Abstract: Applying antibiotics to susceptible bacterial cultures generates a minor population of persisters that remain susceptible to antibiotics but can endure them for extended periods. Recent reports suggest that antibiotic persisters (APs) of mycobacteria experience oxidative… read more here.
Keywords: dnae2; error prone; resistance; prone polymerase ... See more keywords
Thermostability enhancement of Escherichia coli phytase by error-prone polymerase chain reaction (epPCR) and site-directed mutagenesis
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2023.1167530
Abstract: Phytase efficiently hydrolyzes phytate to phosphate; thus, it is widely used to increase phosphorus availability in animal feeds and reduce phosphorus pollution through excretion. Phytase is easily inactivated during feed pelleting at high temperature, and… read more here.
Keywords: polymerase chain; escherichia coli; error prone; phytase ... See more keywords