Characterizing the effects of thermal treatment on human norovirus GII.4 viability using propidium monoazide combined with RT-qPCR and quality assessments in mussels
Sign Up to like & getrecommendations! Published in 2020 at "Food Control"
DOI: 10.1016/j.foodcont.2019.106954
Abstract: Abstract This study investigated the effects of thermal treatment (70, 80, and 90 °C for 5, 10, and 20 min) on the titers of human norovirus (HuNoV) GII.4, a dominant genotype in fresh mussels using propidium monoazide… read more here.
Keywords: propidium monoazide; human norovirus; using propidium; effects thermal ... See more keywords
Propidium Monoazide is Unreliable for Quantitative Live–Dead Molecular Assays
Sign Up to like & getrecommendations! Published in 2025 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.4c05593
Abstract: Propidium monoazide (PMA) is a dye that distinguishes between live and dead cells in molecular assays like the Polymerase Chain Reaction (PCR). It works by cross-linking to the DNA of cells that have compromised membranes… read more here.
Keywords: pma; live dead; molecular assays; propidium monoazide ... See more keywords
Ethidium and propidium monoazide: comparison of potential toxicity on Vibrio sp. viability
Sign Up to like & getrecommendations! Published in 2020 at "Letters in Applied Microbiology"
DOI: 10.1111/lam.13412
Abstract: Vibrio sp., ubiquitous in the aquatic ecosystem, are bacteria of interest because of their involvement in human health, causing gastroenteritis after ingestion of seafood, as well as their role in vibriosis leading to severe losses… read more here.
Keywords: propidium monoazide; ethidium propidium; vibrio; vibrio cells ... See more keywords
The effects of removing dead bacteria by propidium monoazide on the profile of salivary microbiome
Sign Up to like & getrecommendations! Published in 2021 at "BMC Oral Health"
DOI: 10.1186/s12903-021-01832-5
Abstract: Background Oral microbiome played an important role in maintaining healthy state and might exhibit certain changes under circumstances of diseases. However, current microbiological research using sequencing techniques did not regard dead bacteria as a separate… read more here.
Keywords: propidium monoazide; dead bacteria; salivary microbiome; bacteria propidium ... See more keywords
Applicability of propidium monoazide (PMA) for discrimination between living and dead phytoplankton cells
Sign Up to like & getrecommendations! Published in 2019 at "PLoS ONE"
DOI: 10.1371/journal.pone.0218924
Abstract: Propidium monoazide (PMA) is a highly selective dye that penetrates only membrane-compromised, dead microbial cells and inhibits both DNA extraction and amplification. PMA has been widely used for discrimination between living and dead microbial cells;… read more here.
Keywords: pma; living dead; propidium monoazide; applicability ... See more keywords
Detection of suid herpesvirus 1 infectivity in pigs by propidium monoazide-qPCR
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Veterinary Science"
DOI: 10.3389/fvets.2022.975726
Abstract: At present, there is no effective experimental method for detecting whether the suid herpesvirus 1 (SHV-1) detected in pigs is infectious. Although the technique of quantitative polymerase chain reaction (qPCR) has significantly improved the detection… read more here.
Keywords: pma; suid herpesvirus; infectivity; detection ... See more keywords
A Rapid and Reliable Propidium Monoazide Polymerase Chain Reaction for Detecting Viable Pseudomonas syringae pv. actinidiae
Sign Up to like & getrecommendations! Published in 2025 at "Current Issues in Molecular Biology"
DOI: 10.3390/cimb47020103
Abstract: Pseudomonas syringae pv. actinidiae (Psa) is responsible for causing kiwifruit canker disease. The detection of Psa is commonly carried out using normal PCR and culture-based isolation. However, normal PCR does not differentiate between live and… read more here.
Keywords: propidium monoazide; psa; pseudomonas syringae; syringae actinidiae ... See more keywords
Inactivation of Human Norovirus GII.4’s Infectivity in Fresh Oysters (Crassostrea gigas) through Thermal Treatment in Association with Propidium Monoazide
Sign Up to like & getrecommendations! Published in 2024 at "Viruses"
DOI: 10.3390/v16010110
Abstract: The current study investigated the effects of heat treatment (85 °C or 100 °C for 5–20 min) on human norovirus (HuNoV) GII.4’s capsid stability in fresh oysters. In addition, propidium monoazide (PMA) was used in… read more here.
Keywords: treatment; human norovirus; heat; heat treatment ... See more keywords