GST‐IVTT pull‐down: a fast and versatile in vitro method for validating and mapping protein–protein interactions
Sign Up to like & getrecommendations! Published in 2022 at "FEBS Open Bio"
DOI: 10.1002/2211-5463.13485
Abstract: Over the past few decades, dozens of in vitro methods have been developed to map, investigate and validate protein–protein interactions. However, most of these approaches are time‐consuming and labour‐intensive or require specialised equipment or substantial amounts… read more here.
Keywords: protein protein; gst ivtt; fast versatile; method ... See more keywords
A Bionic‐Homodimerization Strategy for Optimizing Modulators of Protein–Protein Interactions: From Statistical Mechanics Theory to Potential Clinical Translation
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DOI: 10.1002/advs.202105179
Abstract: Emerging protein–protein interaction (PPI) modulators have brought out exciting ability as therapeutics in human diseases, but its clinical translation has been greatly hampered by the limited affinity. Inspired by the homodimerize structure of antibody, the… read more here.
Keywords: protein protein; clinical translation; affinity; homodimerization ... See more keywords
Editorial to the Special Issue: “Biochemistry of Protein–Protein and Protein–Lipid Interactions: Applications to Biotechnology”
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DOI: 10.1002/bab.1643
Abstract: This special issue of Biotechnology and Applied Biochemistry puts together original research articles, state-of-the-art reviews, and expert opinions by well-recognized experts on the mechanisms and impact of protein–protein and protein–ligand interactions. All contributors underline that… read more here.
Keywords: biochemistry; special issue; editorial special; protein protein ... See more keywords
A simplified direct on‐chip forward or reverse immunoassay for evaluating protein–protein interactions in the serum
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DOI: 10.1002/biot.202200427
Abstract: The identification of protein–protein interactions is a great challenge. In this study, we fabricated a gold surface‐modified biochip with activated sophorolipids (SLs) in combination with 16‐amino‐1‐hexadecanethiol hydrochloride to detect serum proteins. read more here.
Keywords: protein protein; direct chip; simplified direct; chip forward ... See more keywords
Visualizing the Adenylation Activities and Protein–Protein Interactions of Aryl Acid Adenylating Enzymes
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DOI: 10.1002/cbic.201700361
Abstract: Structural and activity studies have revealed the dynamic and transient actions of carrier protein (CP) activity in primary and secondary metabolic pathways. CP‐mediated interactions play a central role in nonribosomal peptide biosynthesis, as they serve… read more here.
Keywords: protein; protein interactions; aryl acid; protein protein ... See more keywords
Multivalent Ligands with Tailor‐Made Anion Binding Motif as Stabilizers of Protein–Protein Interactions
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DOI: 10.1002/cbic.201900288
Abstract: Modulation of protein–protein interactions (PPIs) is essential for understanding and tuning biologically relevant processes. Although inhibitors for PPIs are widely used, the field still lacks the targeted design of stabilizers. Here, we report unnatural stabilizers… read more here.
Keywords: made anion; ligands tailor; protein interactions; multivalent ligands ... See more keywords
Selective Disruption of Survivin's Protein‐Protein Interactions: A Supramolecular Approach Based on Guanidiniocarbonylpyrrole
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DOI: 10.1002/cbic.202100618
Abstract: Targeting specific protein binding sites to interfere with protein‐protein interactions (PPIs) is crucial for the rational modulation of biologically relevant processes. Survivin, which is highly overexpressed in most cancer cells and considered to be a… read more here.
Keywords: protein protein; selective disruption; disruption survivin; protein interactions ... See more keywords
Proteomic Tools for the Quantitative Analysis of Artificial Peptide Libraries: Detection and Characterization of Target‐Amplified PD‐1 Inhibitors
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DOI: 10.1002/cbic.202200152
Abstract: We report a quantitative proteomics data analysis pipeline, which coupled to protein‐directed dynamic combinatorial chemistry (DDC) experiments, enables the rapid discovery and direct characterization of protein‐protein interaction (PPI) modulators. A low‐affinity PD‐1 binder was incubated… read more here.
Keywords: protein protein; target; peptide; analysis ... See more keywords
Protein‐Protein Recognition Involved in the Intermodular Transacylation Reaction in Modular Polyketide Synthase in the Biosynthesis of Vicenistatin
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DOI: 10.1002/cbic.202200200
Abstract: The ketosynthase (KS) domain is a core domain found in modular polyketide synthases (PKSs). To maintain the polyketide biosynthetic fidelity, the KS domain must only accept an acyl group from the acyl carrier protein (ACP)… read more here.
Keywords: protein protein; domain; transacylation; transacylation reaction ... See more keywords
Chemical Space Overlap with Critical Protein–Protein Interface Residues in Commercial and Specialized Small‐Molecule Libraries
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DOI: 10.1002/cmdc.201800537
Abstract: There is growing interest in the use of structure‐based virtual screening to identify small molecules that inhibit challenging protein–protein interactions (PPIs). In this study, we investigated how effectively chemical library members docked at the PPI… read more here.
Keywords: interface; small molecule; small molecules; protein protein ... See more keywords
Optimization of a Class of Dihydrobenzofurane Analogs toward Orally Efficacious YAP‐TEAD Protein‐Protein Interaction Inhibitors
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DOI: 10.1002/cmdc.202300051
Abstract: The inhibition of the YAP‐TEAD protein‐protein interaction constitutes a promising therapeutic approach for the treatment of cancers linked to the dysregulation of the Hippo signaling pathway. The identification of a class of small molecules which… read more here.
Keywords: interaction; protein protein; protein interaction; class ... See more keywords