Discovering Protein-Protein Interactions Using Nucleic Acid Programmable Protein Arrays.
Sign Up to like & getrecommendations! Published in 2017 at "Current protocols in cell biology"
DOI: 10.1002/cpcb.14
Abstract: We have developed a protocol enabling the study of protein-protein interactions (PPIs) at the proteome level using in vitro-synthesized proteins. Assay preparation requires molecular cloning of the query gene into a vector that supports in… read more here.
Keywords: protein; protein interactions; protein arrays; protein protein ... See more keywords
Discovery of Immune Reactive Human Proteins by High-Density Protein Arrays and Customized Validation of Potential Biomarkers by ELISA.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/7651_2017_92
Abstract: Because of our access to human genome data and ever-improving genome sequencing and proteome analysis methods, we are much better in terms of our understanding of biological processes. In addition to genomics, proteomics, and other… read more here.
Keywords: density protein; protein arrays; high density; discovery immune ... See more keywords
Reverse Phase Protein Arrays.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1064-0_9
Abstract: Reverse phase protein arrays (RPPA) are used to quantify proteins and protein posttranslational modifications in cellular lysates and body fluids. RPPA technology is suitable for biomarker discovery, protein pathway profiling, functional phenotype analysis, and drug… read more here.
Keywords: protein; protein arrays; antibody; reverse phase ... See more keywords
Lysate Preparation for Reverse Phase Protein Arrays.
Sign Up to like & getrecommendations! Published in 2019 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-981-32-9755-5_2
Abstract: Protein lysates from a variety of sample materials, e.g., cell lines, serum, frozen tissues, formalin-fixed and paraffin-embedded (FFPE) tissues, and fine needle aspirates, are compatible with the analysis using reverse phase protein arrays (RPPAs). Due… read more here.
Keywords: protein; phase; lysate preparation; protein arrays ... See more keywords
The effects of sample handling on proteomics assessed by reverse phase protein arrays (RPPA): Functional proteomic profiling in leukemia.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of proteomics"
DOI: 10.1016/j.jprot.2020.104046
Abstract: Reverse phase protein arrays (RPPA) can assess protein expression and activation states in large numbers of samples (n > 1000) and evidence suggests feasibility in the setting of multi-institution clinical trials. Despite evidence in solid tumors, little… read more here.
Keywords: protein; phase; protein arrays; reverse phase ... See more keywords
Multilayered Ordered Protein Arrays Self-Assembled from a Mixed Population of Virus-like Particles.
Sign Up to like & getrecommendations! Published in 2022 at "ACS nano"
DOI: 10.1021/acsnano.1c11272
Abstract: Biology shows many examples of spatially controlled assembly of cells and biomacromolecules into hierarchically organized structures, to which many of the complex biological functions are attributed. While such biological structures have inspired the design of… read more here.
Keywords: ordered protein; arrays self; like particles; protein arrays ... See more keywords
Application of mRNA Arrays for the Production of mCherry Reporter-Protein Arrays for Quantitative Gene Expression Analysis.
Sign Up to like & getrecommendations! Published in 2019 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.8b00266
Abstract: The development of programmable regulators that precisely and predictably control gene expression is a major goal of synthetic biology. Consequently, rapid high-throughput biochemical methods capable of quantitatively analyzing all components of gene expression would be… read more here.
Keywords: protein arrays; gene expression; biology; gene ... See more keywords