Mass spectrometry-A versatile tool for characterising the lipid environment of membrane protein assemblies.
Sign Up to like & getrecommendations! Published in 2019 at "Chemistry and physics of lipids"
DOI: 10.1016/j.chemphyslip.2019.04.001
Abstract: Biological membranes are selectively permeable barriers important for cell organization and compartmentalization. Their organisation strongly depends on the lipids that constitute the lipid bilayer as well as the proteins that reside in the membrane. Unravelling… read more here.
Keywords: mass spectrometry; lipid environment; membrane protein; protein assemblies ... See more keywords
Computational modeling of protein assemblies.
Sign Up to like & getrecommendations! Published in 2017 at "Current opinion in structural biology"
DOI: 10.1016/j.sbi.2017.04.006
Abstract: Computational methods to predict the 3D structures of protein interactions fall into 3 categories-template based modeling, protein-protein docking and hybrid/integrative modeling. The two most important considerations for modeling methods are sampling and scoring conformations. Sampling… read more here.
Keywords: protein; modeling protein; microscopy; computational modeling ... See more keywords
The computational prediction of protein assemblies.
Sign Up to like & getrecommendations! Published in 2017 at "Current opinion in structural biology"
DOI: 10.1016/j.sbi.2017.10.006
Abstract: The function of proteins in the cell is almost always mediated by their interaction with different partners, including other proteins, nucleic acids or small organic molecules. The ability of identifying all of them is an… read more here.
Keywords: protein; protein assemblies; prediction protein; computational prediction ... See more keywords
High-symmetry protein assemblies: patterns and emerging applications.
Sign Up to like & getrecommendations! Published in 2019 at "Current opinion in structural biology"
DOI: 10.1016/j.sbi.2019.03.008
Abstract: The accelerated elucidation of three-dimensional structures of protein complexes, both natural and designed, is providing new examples of large supramolecular assemblies with intriguing shapes. Those with high symmetry - based on the geometries of the… read more here.
Keywords: assemblies patterns; symmetry protein; high symmetry; protein assemblies ... See more keywords
FAIMS and native mass spectrometry: Analysis of intact protein assemblies and protein complexes.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c00649
Abstract: High-field asymmetric waveform ion mobility spectrometry (FAIMS) enables the separation of ions on the basis of their differential mobility in an asymmetric oscillating electric field. We, and others, have previously demonstrated the benefits of FAIMS… read more here.
Keywords: native mass; spectrometry; mass spectrometry; analysis ... See more keywords
Native Ambient Mass Spectrometry Enables Analysis of Intact Endogenous Protein Assemblies up to 145 kDa Directly from Tissue
Sign Up to like & getrecommendations! Published in 2022 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.1c05353
Abstract: Untargeted label-free interrogation of proteins in their functional form directly from their physiological environment promises to transform life sciences research by providing unprecedented insight into their transient interactions with other biomolecules and xenobiotics. Native ambient… read more here.
Keywords: mass spectrometry; endogenous protein; protein assemblies;
Design of metal-mediated protein assemblies via hydroxamic acid functionalities.
Sign Up to like & getrecommendations! Published in 2021 at "Nature protocols"
DOI: 10.1038/s41596-021-00535-z
Abstract: The self-assembly of proteins into sophisticated multicomponent assemblies is a hallmark of all living systems and has spawned extensive efforts in the construction of novel synthetic protein architectures with emergent functional properties. Protein assemblies in… read more here.
Keywords: protein; construction; protein assemblies; metal ... See more keywords
On the formation of ordered protein assemblies in cell–cell interfaces
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2206175119
Abstract: Significance This paper discusses simulation techniques used to study the formation of organized protein assemblies on cell–cell interfaces. The simulations emphasize the need for a realistic representation of protein structure when studying such cellular phenomena.… read more here.
Keywords: assemblies cell; cell interfaces; formation; cell cell ... See more keywords
Engineering protein assemblies with allosteric control via monomer fold-switching.
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.2210/pdb6qiz/pdb
Abstract: The macromolecular machines of life use allosteric control to self-assemble, dissociate and change shape in response to signals. Despite enormous interest, the design of nanoscale allosteric assemblies has proven tremendously challenging. Here we present a… read more here.
Keywords: engineering protein; monomer; protein assemblies; allosteric control ... See more keywords
Reversible protein assemblies in the proteostasis network in health and disease
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2023.1155521
Abstract: While proteins populating their native conformations constitute the functional entities of cells, protein aggregates are traditionally associated with cellular dysfunction, stress and disease. During recent years, it has become clear that large aggregate-like protein condensates… read more here.
Keywords: disease; assemblies proteostasis; reversible protein; health disease ... See more keywords
The Versatile Manipulations of Self-Assembled Proteins in Vaccine Design
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms22041934
Abstract: Protein assemblies provide unique structural features which make them useful as carrier molecules in biomedical and chemical science. Protein assemblies can accommodate a variety of organic, inorganic and biological molecules such as small proteins and… read more here.
Keywords: protein; protein assemblies; versatile manipulations; structural features ... See more keywords