Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL)
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Extracellular Vesicles"
DOI: 10.1002/jev2.12245
Abstract: Abstract Extracellular vesicles (EVs) are blood‐borne messengers that coordinate signalling between different tissues and organs in the body. The specificity of such crosstalk is determined by preferential EV docking to target sites, as mediated through… read more here.
Keywords: protein complexes; extracellular vesicles; extracellular vesicle; intact extracellular ... See more keywords
A Novel Core‐Attachment–Based Method to Identify Dynamic Protein Complexes Based on Gene Expression Profiles and PPI Networks
Sign Up to like & getrecommendations! Published in 2019 at "PROTEOMICS"
DOI: 10.1002/pmic.201800129
Abstract: Cellular functions are always performed by protein complexes. At present, many approaches have been proposed to identify protein complexes from protein–protein interaction (PPI) networks. Some approaches focus on detecting local dense subgraphs in PPI networks… read more here.
Keywords: protein; ppi networks; method; expression ... See more keywords
Dockground: A comprehensive data resource for modeling of protein complexes
Sign Up to like & getrecommendations! Published in 2018 at "Protein Science"
DOI: 10.1002/pro.3295
Abstract: Characterization of life processes at the molecular level requires structural details of protein interactions. The number of experimentally determined structures of protein–protein complexes accounts only for a fraction of known protein interactions. This gap in… read more here.
Keywords: protein; modeling protein; protein interactions; resource ... See more keywords
Preparation of SNAPf-Beads for Colocalization Single-Molecule Spectroscopy (CoSMoS) of RNA-Protein Complexes.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0278-2_2
Abstract: The SNAPf-tag is a chemical tag that allows rapid and highly specific covalent labeling of proteins even in the full complexity of the cellular environment. The SNAPf-tag has been instrumental to study native RNA-protein complexes… read more here.
Keywords: snapf; single molecule; protein complexes; spectroscopy ... See more keywords
System-Wide Profiling of Protein Complexes Via Size Exclusion Chromatography-Mass Spectrometry (SEC-MS).
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1178-4_18
Abstract: In living cells, most proteins are organized in stable or transient functional assemblies, protein complexes, which control a multitude of vital cellular processes such as cell cycle progression, metabolism, and signal transduction. Over several decades,… read more here.
Keywords: protein; mass spectrometry; analysis; biology ... See more keywords
Methods for Molecular Modelling of Protein Complexes.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1406-8_3
Abstract: Biological processes are often mediated by complexes formed between proteins and various biomolecules. The 3D structures of such protein-biomolecule complexes provide insights into the molecular mechanism of their action. The structure of these complexes can… read more here.
Keywords: protein; complexes methods; methods molecular; molecular modelling ... See more keywords
Combining Electron Microscopy (EM) and Cross-Linking Mass Spectrometry (XL-MS) for Structural Characterization of Protein Complexes.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1936-0_17
Abstract: Structural biology has recently witnessed the benefits of the combined use of two complementary techniques: electron microscopy (EM) and cross-linking mass spectrometry (XL-MS). EM (especially its cryogenic variant cryo-EM) has proven to be a very… read more here.
Keywords: protein complexes; electron microscopy; microscopy; characterization ... See more keywords
Single-Cell Single-Molecule Pull-Down (sc-SiMPull) for Detection of Protein Complexes from Embryonic Lysates.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2035-9_4
Abstract: Mapping how proteins form complexes and change binding partners is central to understanding cell signaling. Bulk biochemistry can provide a summary of what complexes are present in a cell, but information about the diversity of… read more here.
Keywords: protein complexes; single cell; cell; molecule pull ... See more keywords
Isolation of Native Soluble and Membrane-Bound Protein Complexes from Yeast Saccharomyces cerevisiae.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6937-1_4
Abstract: Immunoprecipitation is a traditional approach to isolate single proteins or native protein complexes from a complex sample mixture. The original method makes use of specific antibodies against endogenous proteins or epitope tags, which are first… read more here.
Keywords: protein; yeast saccharomyces; isolation native; saccharomyces cerevisiae ... See more keywords
Considerations for Identifying Endogenous Protein Complexes from Tissue via Immunoaffinity Purification and Quantitative Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9232-4_9
Abstract: Protein complexes perform key roles in nearly all aspects of biology. Identification of the composition of these complexes offers insights into how different cellular processes are carried out. The use of affinity purification coupled to… read more here.
Keywords: protein; purification; mass spectrometry; protein complexes ... See more keywords
Electrophoretic Mobility Shift Assay and Dimethyl Sulfate Footprinting for Characterization of G-Quadruplexes and G-Quadruplex-Protein Complexes.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9666-7_11
Abstract: DNA G-quadruplexes are globular nucleic acid secondary structures which occur throughout the human genome under physiological conditions. There is accumulating evidence supporting G-quadruplex involvement in a number of important aspects of genome functions, including transcription,… read more here.
Keywords: protein; electrophoretic mobility; characterization quadruplexes; quadruplex protein ... See more keywords