On the design of a constitutively active peptide asparaginyl ligase for facile protein conjugation.
Sign Up to like & getrecommendations! Published in 2023 at "FEBS open bio"
DOI: 10.1002/2211-5463.13575
Abstract: Peptide asparaginyl ligases (PALs) are precision tools for peptide cyclization, cell‐surface labelling, protein semisynthesis and protein conjugation. PALs are expressed as inactive proenzymes requiring low pH activation. During activation, a large portion of the cap… read more here.
Keywords: ligase; peptide asparaginyl; protein conjugation; constitutively active ... See more keywords
Characterization of High Molecular Weight Multi-arm Functionalized PEG-Maleimide for Protein Conjugation by Charge Reduction Mass Spectrometry Coupled to Two-Dimensional Liquid Chromatography.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c01567
Abstract: A current trend in drug development involves the use of high molecular weight, branched and functionalized polymers for protein conjugation and drug delivery. Accurately characterizing these polymers is critical to control product quality, monitor stability,… read more here.
Keywords: protein conjugation; mass; charge; high molecular ... See more keywords
Ligand-Binding Cooperativity Effects in Polymer-Protein Conjugation.
Sign Up to like & getrecommendations! Published in 2019 at "Biomacromolecules"
DOI: 10.1021/acs.biomac.9b00016
Abstract: We present an electron paramagnetic resonance (EPR) spectroscopic characterization of structural and dynamic effects that stem from post-translational modifications of bovine serum albumin (BSA), an established model system for polymer-protein conjugation. Beyond the typical drug… read more here.
Keywords: protein conjugation; polymer protein; spectroscopy; ligand binding ... See more keywords