Recent experimental and theoretical studies on protein crystallization
Sign Up to like & getrecommendations! Published in 2017 at "Crystal Research and Technology"
DOI: 10.1002/crat.201600210
Abstract: Crystals are required to determine three-dimensional structures of proteins and other bio-molecules via X-ray diffraction. This is the prime cause for the keen interest in protein crystallization. Recently published experimental and theoretical studies on protein… read more here.
Keywords: theoretical studies; crystallization; studies protein; experimental theoretical ... See more keywords
Characterization of New Detergents and Detergent Mimetics by Scattering Techniques for Membrane Protein Crystallization.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7151-0_9
Abstract: Membrane proteins are difficult to manipulate and stabilize once they have been removed from their native membranes. However, despite these difficulties, successes in membrane-protein structure determination have continued to accumulate for over two decades, thanks… read more here.
Keywords: scattering techniques; crystallization; detergent; membrane protein ... See more keywords
Microfluidic Technologies and Platforms for Protein Crystallography
Sign Up to like & getrecommendations! Published in 2019 at "Bioanalysis"
DOI: 10.1007/978-981-13-6229-3_2
Abstract: Protein crystallization and its three-dimensional structure analysis is indispensable for understanding the protein function in the body and life phenomenon. Three dimensional structure of protein also plays important role for drug discovery and it have… read more here.
Keywords: protein; three dimensional; technologies platforms; protein crystallization ... See more keywords
Protein crystallization in a droplet-based microfluidic device: Hydrodynamic analysis and study of the phase behaviour
Sign Up to like & getrecommendations! Published in 2018 at "Chemical Engineering Science"
DOI: 10.1016/j.ces.2018.06.066
Abstract: S.K. acknowledges funding from the European Research Council under the ERC Starting Grant Agreement no. 677169–MicroParticleControl. F.C acknowledges FCT (postdoctoral fellowship [SFRH/BPD/96132/2013]) under the project POCI-01-0145-FEDER-006939 (Laboratory for Process Engineering, Environment, Biotechnology and Energy –… read more here.
Keywords: crystallization droplet; device hydrodynamic; droplet based; microfluidic device ... See more keywords
An investigation on the effect of surface roughness of crystallization plate on protein crystallization
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Crystal Growth"
DOI: 10.1016/j.jcrysgro.2016.10.007
Abstract: Abstract Surface treatment by oxidizing the crystallization plates can significantly promote protein crystallization and requires no change to routine screening protocols; therefore, it is potentially useful for practical protein crystallization screening. However, experiments have shown… read more here.
Keywords: surface treatment; protein crystallization; crystallization;
Expanding pH screening space using multiple droplets with secondary buffers for protein crystallization
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Crystal Growth"
DOI: 10.1016/j.jcrysgro.2017.01.036
Abstract: Abstract We have proposed a rational strategy for selecting a suitable pH of protein solution based on protein biochemical properties. However, it is difficult to use this strategy for biochemical properties unknown proteins. In this… read more here.
Keywords: expanding screening; screening space; crystallization; strategy ... See more keywords
Hydrophobic Interface-Assisted Protein Crystallization: Theory and Experiment.
Sign Up to like & getrecommendations! Published in 2019 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.8b20995
Abstract: Macromolecular crystallization is crucial to a large number of scientific fields, including structural biology; drug design, formulation, and delivery; manufacture of biomaterials; and preparation of foodstuffs. The purpose of this study is to facilitate control… read more here.
Keywords: crystallization; interface assisted; nucleation; hydrophobic interface ... See more keywords
Characterization of amyloid β fibril formation under microgravity conditions
Sign Up to like & getrecommendations! Published in 2020 at "NPJ Microgravity"
DOI: 10.1038/s41526-020-0107-y
Abstract: Amyloid fibrils are self-assembled and ordered proteinaceous supramolecules structurally characterized by the cross-β spine. Amyloid formation is known to be related to various diseases typified by neurogenerative disorders and involved in a variety of functional… read more here.
Keywords: microgravity conditions; formation; fibril formation; microgravity ... See more keywords
A review of solvent freeze-out technology for protein crystallization
Sign Up to like & getrecommendations! Published in 2021 at "CrystEngComm"
DOI: 10.1039/d1ce00005e
Abstract: The demand for proteins with high purity, high activity and high stability is increasing, especially in the biopharmaceutical industry. Traditional industrial protein production technologies include chromatography, which is expensive, and salting out and the traditional… read more here.
Keywords: technology protein; protein crystallization; solvent freeze; protein ... See more keywords
SADeepcry: a deep learning framework for protein crystallization propensity prediction using self-attention and auto-encoder networks
Sign Up to like & getrecommendations! Published in 2022 at "Briefings in bioinformatics"
DOI: 10.1093/bib/bbac352
Abstract: The X-ray diffraction (XRD) technique based on crystallography is the main experimental method to analyze the three-dimensional structure of proteins. The production process of protein crystals on which the XRD technique relies has undergone multiple… read more here.
Keywords: protein crystallization; crystallization propensity; crystallization; framework ... See more keywords
Critical evaluation of bioinformatics tools for the prediction of protein crystallization propensity
Sign Up to like & getrecommendations! Published in 2017 at "Briefings in bioinformatics"
DOI: 10.1093/bib/bbx076
Abstract: X-ray crystallography is the main tool for structural determination of proteins. Yet, the underlying crystallization process is costly, has a high attrition rate and involves a series of trial-and-error attempts to obtain diffraction-quality crystals. The… read more here.
Keywords: crystallization propensity; critical evaluation; protein crystallization; crystallization ... See more keywords