Protein Design with Fluoroprolines: 4,4‐Difluoroproline Does Not Eliminate the Rate‐Limiting Step of Thioredoxin Folding
Sign Up to like & getrecommendations! Published in 2021 at "ChemBioChem"
DOI: 10.1002/cbic.202100418
Abstract: C4‐substituted fluoroprolines (4R)‐fluoroproline ((4R)‐Flp) and (4S)‐fluoroproline ((4S)‐Flp) have been used in protein engineering to enhance the thermodynamic stability of peptides and proteins. The electron‐withdrawing effect of fluorine can bias the pucker of the pyrrolidine ring,… read more here.
Keywords: rate limiting; protein design; rate; peptide bond ... See more keywords
Rational and Semirational Protein Design.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7366-8_2
Abstract: This mini review gives an overview over different design approaches and methodologies applied in rational and semirational enzyme engineering. The underlying principles for engineering novel activities, enantioselectivity, substrate specificity, stability, and pH optimum are summarized. read more here.
Keywords: rational semirational; design; protein design; biology ... See more keywords
Outer membrane protein design.
Sign Up to like & getrecommendations! Published in 2017 at "Current opinion in structural biology"
DOI: 10.1016/j.sbi.2016.11.003
Abstract: Membrane proteins are the gateway to the cell. These proteins are also a control center of the cell, as information from the outside is passed through membrane proteins as signals to the cellular machinery. The… read more here.
Keywords: membrane proteins; outer membrane; protein design; membrane ... See more keywords
Towards descriptor of elementary functions for protein design.
Sign Up to like & getrecommendations! Published in 2019 at "Current opinion in structural biology"
DOI: 10.1016/j.sbi.2019.06.010
Abstract: We review studies of the protein evolution that help to formulate rules for protein design. Acknowledging the fundamental importance of Dayhoff's provision on the emergence of functional proteins from short peptides, we discuss multiple evidences… read more here.
Keywords: elementary functions; protein design; towards descriptor; descriptor elementary ... See more keywords
Deep learning techniques have significantly impacted protein structure prediction and protein design.
Sign Up to like & getrecommendations! Published in 2021 at "Current opinion in structural biology"
DOI: 10.1016/j.sbi.2021.01.007
Abstract: Protein structure prediction and design can be regarded as two inverse processes governed by the same folding principle. Although progress remained stagnant over the past two decades, the recent application of deep neural networks to… read more here.
Keywords: protein; protein structure; protein design; structure prediction ... See more keywords
Arming Yourself for The In Silico Protein Design Revolution.
Sign Up to like & getrecommendations! Published in 2020 at "Trends in biotechnology"
DOI: 10.1016/j.tibtech.2020.10.003
Abstract: Proteins mediate many essential processes of life to a degree of functional precision unmatched by any synthetic device. While engineered proteins are currently used in biotech, food, biomedicine, and material technology-based industries, the true potential… read more here.
Keywords: protein; design revolution; arming silico; protein design ... See more keywords
Harnessing Human Neural Networks for Protein Design.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemistry"
DOI: 10.1021/acs.biochem.9b00820
Abstract: C proteins to fulfill a variety of functional needs has long been a goal of biochemists. This requires a thorough understanding of the relationship between the sequence of a polypeptide chain and the resulting protein… read more here.
Keywords: protein; foldit; protein design; structure ... See more keywords
Protein Design: From the Aspect of Water Solubility and Stability
Sign Up to like & getrecommendations! Published in 2022 at "Chemical Reviews"
DOI: 10.1021/acs.chemrev.1c00757
Abstract: Water solubility and structural stability are key merits for proteins defined by the primary sequence and 3D-conformation. Their manipulation represents important aspects of the protein design field that relies on the accurate placement of amino… read more here.
Keywords: protein design; solubility; water solubility; design ... See more keywords
Computational Protein Design for COVID-19 Research and Emerging Therapeutics
Sign Up to like & getrecommendations! Published in 2023 at "ACS Central Science"
DOI: 10.1021/acscentsci.2c01513
Abstract: As the world struggles with the ongoing COVID-19 pandemic, unprecedented obstacles have continuously been traversed as new SARS-CoV-2 variants continually emerge. Infectious disease outbreaks are unavoidable, but the knowledge gained from the successes and failures… read more here.
Keywords: computational protein; protein design; design covid; covid research ... See more keywords
Protein design and variant prediction using autoregressive generative models
Sign Up to like & getrecommendations! Published in 2021 at "Nature Communications"
DOI: 10.1038/s41467-021-22732-w
Abstract: The ability to design functional sequences and predict effects of variation is central to protein engineering and biotherapeutics. State-of-art computational methods rely on models that leverage evolutionary information but are inadequate for important applications where… read more here.
Keywords: prediction using; design variant; protein design; variant prediction ... See more keywords
An adapted consensus protein design strategy for identifying globally optimal biotherapeutics
Sign Up to like & getrecommendations! Published in 2022 at "mAbs"
DOI: 10.1080/19420862.2022.2073632
Abstract: ABSTRACT Biotherapeutic optimization, whether to improve general properties or to engineer specific attributes, is a time-consuming process with uncertain outcomes. Conversely, Consensus Protein Design has been shown to be a viable approach to enhance protein… read more here.
Keywords: protein design; consensus; adapted consensus; consensus protein ... See more keywords