Light Sheet Microscopy to Measure Protein Dynamics
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Cellular Physiology"
DOI: 10.1002/jcp.25451
Abstract: Visualizing protein dynamics is the key to a quantitative understanding of molecular mechanisms in biological systems. Recent developments in fluorescent microscopy techniques allow for novel experimental approaches to study stochastic localization, distribution, and movement of… read more here.
Keywords: protein dynamics; microscopy; measure protein; light sheet ... See more keywords
The Effects of Protein Dynamics on Immune System Signaling Pathways
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.380
Abstract: Activation of signaling pathways in immune cells can be initiated by binding of membrane proteins, known as B-cell receptors (BCRs) or T-cell receptors (TCRs), to fragments of an antigen. The proteins spleen tyrosine kinase (Syk)… read more here.
Keywords: signaling pathways; protein dynamics; system signaling; effects protein ... See more keywords
Protein Dynamics Enables Phosphorylation of Buried Residues in Cdk2/Cyclin-A-Bound p27.
Sign Up to like & getrecommendations! Published in 2020 at "Biophysical journal"
DOI: 10.1016/j.bpj.2020.06.040
Abstract: Proteins carry out a wide range of functions that are tightly regulated in space and time. Protein phosphorylation is the most common post-translation modification of proteins and plays a key role in the regulation of… read more here.
Keywords: protein dynamics; phosphorylation buried; buried residues; phosphorylation ... See more keywords
Tier-0 protein dynamics of bovine serum albumin: A kinetics and energetics study of the collective domain motions
Sign Up to like & getrecommendations! Published in 2021 at "Chemical Physics Letters"
DOI: 10.1016/j.cplett.2020.138102
Abstract: Abstract The time-resolved confocal fluorescent thermometry coupled with a reversible two-state model were employed to characterize the thermally-induced dynamics of bovine serum albumin (BSA) on a millisecond timescale. Fluorescence intensity change of the constituent tryptophans… read more here.
Keywords: protein dynamics; dynamics bovine; tier protein; serum albumin ... See more keywords
Prediction of disordered regions in proteins with recurrent Neural Networks and protein dynamics.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of molecular biology"
DOI: 10.1016/j.jmb.2022.167579
Abstract: The role of intrinsically disordered protein regions (IDRs) in cellular processes has become increas- ingly evident over the last years. These IDRs continue to challenge structural biology experiments be- cause they lack a well-defined conformation,… read more here.
Keywords: biology; prediction disordered; protein dynamics; disordered regions ... See more keywords
Protein dynamics and conformational changes explored by hydrogen/deuterium exchange mass spectrometry.
Sign Up to like & getrecommendations! Published in 2019 at "Current opinion in structural biology"
DOI: 10.1016/j.sbi.2019.06.007
Abstract: Proteins are not rigid bodies under their physiological conditions. Here we discuss a solution-phase structural proteomics technique, hydrogen deuterium exchange coupled with mass spectrometry (HDX-MS), as a means to study protein dynamics, which can complement… read more here.
Keywords: protein dynamics; deuterium exchange; hydrogen deuterium; mass spectrometry ... See more keywords
Complete Extraction of Protein Dynamics Information in Hydrogen/Deuterium Exchange Mass Spectrometry Data.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.9b05724
Abstract: Hydrogen/deuterium exchange (HDX) mass spectrometry (MS) has been used to study protein conformation and conformational dynamics. A continuous labeling experiment, followed by proteolytic digestion and MS analysis, generates a large amount of data, containing information… read more here.
Keywords: protein dynamics; information; hydrogen deuterium; dynamics information ... See more keywords
The Stories Tryptophans Tell: Exploring Protein Dynamics of Heptosyltransferase I from Escherichia coli.
Sign Up to like & getrecommendations! Published in 2017 at "Biochemistry"
DOI: 10.1021/acs.biochem.6b00850
Abstract: Heptosyltransferase I (HepI) catalyzes the addition of l-glycero-β-d-manno-heptose to Kdo2-Lipid A, as part of the biosynthesis of the core region of lipopolysaccharide (LPS). Gram-negative bacteria with gene knockouts of HepI have reduced virulence and enhanced… read more here.
Keywords: protein dynamics; tryptophans tell; adp heptose; stories tryptophans ... See more keywords
Protein Dynamics and the Folding Degree
Sign Up to like & getrecommendations! Published in 2020 at "Journal of chemical information and modeling"
DOI: 10.1021/acs.jcim.9b00942
Abstract: The analysis of folding trajectories for proteins is an open challenge. One of the problems is how to describe the amount of folded secondary structure in a protein. We extend the use of Estradas' folding… read more here.
Keywords: folding degree; protein; protein dynamics; contribution ... See more keywords
Probing Adaptation of Hydration and Protein Dynamics to Temperature
Sign Up to like & getrecommendations! Published in 2022 at "ACS Omega"
DOI: 10.1021/acsomega.2c02843
Abstract: Protein dynamics is strongly influenced by the surrounding environment and physiological conditions. Here we employ broadband megahertz-to-terahertz spectroscopy to explore the dynamics of water and myoglobin protein on an extended time scale from femto- to… read more here.
Keywords: hydration; water; probing adaptation; tightly bound ... See more keywords
Discrete analyses of protein dynamics
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Biomolecular Structure and Dynamics"
DOI: 10.1080/07391102.2019.1650112
Abstract: Abstract Protein structures are highly dynamic macromolecules. This dynamics is often analysed through experimental and/or computational methods only for an isolated or a limited number of proteins. Here, we explore large-scale protein dynamics simulation to… read more here.
Keywords: protein; protein dynamics; discrete analyses; analyses protein ... See more keywords